DreEX6073705 @ danRer10
Exon Skipping
Gene
ENSDARG00000028533 | macf1a
Description
microtubule-actin crosslinking factor 1a [Source:ZFIN;Acc:ZDB-GENE-030131-3606]
Coordinates
chr19:35552196-35564612:-
Coord C1 exon
chr19:35564477-35564612
Coord A exon
chr19:35554977-35555126
Coord C2 exon
chr19:35552196-35552345
Length
150 bp
Sequences
Splice sites
3' ss Seq
TGACATTTATATTTTTGCAGGAA
3' ss Score
9.11
5' ss Seq
GAGGTAAAA
5' ss Score
7.3
Exon sequences
Seq C1 exon
GCTGTCCCCTGAAGAGCGTGCCCAGGTGGTCTCTCAGCTGGATGAGCTGACGGCAACATACACACAACTGTGTGATAGTTCTGCCGCACAAGTGCAACATCTAGAAGAGCAACTGGCCAAAGAGGAAAAGCGCAAG
Seq A exon
GAAGCTGTAAACAGTCAGCTGAAGTCTTTGCAAGATCATGTTGACAAACTTCTCAGTTGGGTTAAGGAGACTGACACTCAAATGCAGGAAGAAACTGGGGTAGACGGACAGACAGTGGCAGAGATCACTCGGAAGCAGCAGTTGTGTGAG
Seq C2 exon
GTTGTGCAGCAGCAGAAGGCTGAATGTTCTCAAAAACTGGAGAGTCTCACAATGTGGATGGCTGGTGCTGCAAGTCTTTTGGCCAGTCAGAGACCTGGAGTTGAGTCAGATGACGTGCACACATTACAGGAGAAGCAAAGAGCGCTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000028533-'41-53,'41-50,46-53=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.248 A=0.540 C2=0.526
Domain overlap (PFAM):
C1:
PF086146=ATG16=FE(26.3=100),PF087375=Rgp1=FE(34.4=100)
A:
PF0043516=Spectrin=PU(40.2=90.0)
C2:
NO
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGGTCTCTCAGCTGGATGA
R:
GCGCTCTTTGCTTCTCCTGTA
Band lengths:
257-407
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]