DreINT0100271 @ danRer10
Intron Retention
Gene
ENSDARG00000017004 | myo10
Description
myosin X [Source:ZFIN;Acc:ZDB-GENE-070912-277]
Coordinates
chr2:42779085-42779508:-
Coord C1 exon
chr2:42779402-42779508
Coord A exon
chr2:42779310-42779401
Coord C2 exon
chr2:42779085-42779309
Length
92 bp
Sequences
Splice sites
5' ss Seq
ACGGTAAAC
5' ss Score
5.88
3' ss Seq
TTGCTCTCTGCTGGTCACAGGAG
3' ss Score
6.17
Exon sequences
Seq C1 exon
GTGTTTATGAGAGAGAGTTTGGAGCTGCGGTTAGAAAAACAAAGAGAAACAGAAGTTCTCAAAGCAGCATCAATTATCCAAGCTCATATACTGGGCTACAGAGCACG
Seq A exon
GTAAACACACTGGCACGTGCATATACACAAACAAACACTAAAGTACTAAACTCTAATGTAGTCACTGTGTTTTTGCTCTCTGCTGGTCACAG
Seq C2 exon
GAGGCAGTACAGACGCCTGTTGCTGTGCATTGTTGTCATTCAGAAAAATTACCGTGCTCTATATTGGCGCCGACGCTTTCAGACAATGCGATGGGCCACCGTCACCTTACAGAAGAGGCTCAGAGGTCAAAGGGCACGAAGGCTCTATGTTCACCTTTTAGAGGAGAAGAGGAAGAGGGAAGAGGAAGAAAGGAGACACAGAGAAGAACAGGAGGCACTGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017004:ENSDART00000140159:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.171
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(0.5=8.3),PF0061222=IQ=PU(76.2=44.4)
A:
NA
C2:
PF0061222=IQ=PD(19.0=5.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGAGAGAGTTTGGAGCTGCG
R:
GTCGGCGCCAATATAGAGCAC
Band lengths:
173-265
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]