DreINT0108368 @ danRer10
Intron Retention
Gene
ENSDARG00000102825 | olfm2b
Description
olfactomedin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-2402]
Coordinates
chr1:58539266-58541150:-
Coord C1 exon
chr1:58540995-58541150
Coord A exon
chr1:58539486-58540994
Coord C2 exon
chr1:58539266-58539485
Length
1509 bp
Sequences
Splice sites
5' ss Seq
CAGGTACAC
5' ss Score
7.29
3' ss Seq
GTGGCTGTGTCCGTTGACAGGAG
3' ss Score
6.43
Exon sequences
Seq C1 exon
GTGCTGAACGTCTCTGAGCACATGCAGCTCCTGACTCGGCGCACCGCTGGAGATCTGCAGGAGCTCAGAAGCTCGGAGACGATATTGGACACACTGGAGAAAACACTGACCAGTGCCCAGCAGAACCCGCAGGAGCTGAGCGACAGGAGCCTGCAG
Seq A exon
GTACACACACACACACACACACACACGTTTGCATGCAGTGTATATTTTGTATTCATTAGTAGACTGCGGAGTTTCTCTGGTCCACTGTGTCGAGTTATTTCCTAGTGCTTCCAGCAGAGATGCTGAATCTTGAAAACATCCATGAAAGAAAAAGGCAGGAGCCAGCGAGCGCTCAACAATCAACTCTGATCATAAAATAAACACACAACCAAAGTCTCCATCAGGGATTCACCATGCGACTTGAGGCCACTGGCCTAGCTCTGATGTCACAACTTGTGGCCCCGCCCACATCCACAATAATGTTTGATGGCAGCGTCTGCTCATGTGTCAATGTTAAGTTCATGTTGTGAGAGGTGCGCATCAGATCATGCGGGTGGTATGCATCCTTTGGGAAGGTCACACTAGACCTACTACATCCACTTACTGAAGAAGTGTAAATTGGCTCTGACCATCACTACAGAGGGCTTTACCTTTACCGTAAAACTTTAACCTGTATCCTAACTCTTACCCTAAACCCCCTACCCTAGCCCTTACAATGAACCCCTAACTCTTGCTCTTAACTCTAACCCTTACTCTAACACTTACCCTAAAACACTCAACCCTAAACTCTTGCCCTAAACCCTAACCGTTTTCATCACTCTTACCCTAAATTTCTTACTCTAAACCTTAAAATTTACCATAAACCTTACCCAAAACTCCTAACTCTTACCCTGAACTCTTACCTTAAATCCTTATCCTAAACCCCAGAGCCTTACCCTAAATCCCAACCCTTACCCTAAATCCTAACCTTTACCATAACTTTTACCCTAAACCCCTAACTCTTACCCTAAACCCCCAAGCCTTACCCTAAACCCTTACCATTACCCTTTCCCCAAACCCCTAATCCCTAATCTCTAATCCTTACCCTAACCTCTACCTTAAACCTCTAACCCTTACCCCTACCCTGAACTCTTGGCCTAATTACTTACCCTAAACCCCCGAACCTTAGCCTAAATCCCAACTCTTACCCTAAACCCTAACCCTTAGCCTTAACCTTACTGTTACCTTTACCCCAGACCCCTAGTCCCTAACCCTAACGCTTAACCCTTACCATTGCCTTTACCCCAAACCCCAATCCCTAACCCTTATGCCCCATTCACAAGGGCCGTCAGCGTCAACGCTTCCCCTTCACTTTGAATAGGTGACGTCAGGCATTGCCGAACTGCATTGTGCATCCGTCGGCGCCGCTTCAGTGGCATGGCTCGCTGCAAAAGTTGGGACTACCTCAACTTTTTGAGCGCTAACAGAAGCGTCAGCCAATCAGATCGCTGTAATCAAATACTCCTGCTAGACAGTGGCCTATTGCTGACTGAATTTCATTGGCTGACGCTGCTATGACGATCGCTTCAGCCCCAACTTCAGACACGCCTTCAGTCAAGCGTTGACGCTGAAGCCCCGTGGGAATGGAGCGTTACCCTTAAGCCTAACTCTTGCCTCAGTGTCGCCCCCTGTGGCTGTGTCCGTTGACAG
Seq C2 exon
GAGCTGCGCTGGAGTGTGTCTCAGTGTCGCCCCCTGCGGGTGCTGCTGACTCGAGTGCGTGCGGATGTGGCTCAGCTGGACTCTGTGCGTGACGAGCTGCAGGGGATCAGTGTGTCGCTGTCACTGCTGCAGGAACGCTTCACACTCCAGCACTATCAGCAGCTGCAGCAGCGCATCTCCATCCTGCAGCAACACCTGCACAGCTGCTCCAGCCACCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000102825:ENSDART00000168919:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.192 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF123083=Noelin-1=FE(52.0=100),PF127952=MscS_porin=FE(34.9=100)
A:
NA
C2:
PF123083=Noelin-1=PD(0.1=0.0),PF127952=MscS_porin=PD(47.3=93.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACGTCTCTGAGCACATGCAG
R:
GTGTTGCTGCAGGATGGAGAT
Band lengths:
345-1854
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]