DreINT0131571 @ danRer10
Intron Retention
Gene
ENSDARG00000018032 | scn8ab
Description
sodium channel, voltage gated, type VIII, alpha subunit b [Source:ZFIN;Acc:ZDB-GENE-060906-2]
Coordinates
chr6:39400178-39400689:-
Coord C1 exon
chr6:39400405-39400689
Coord A exon
chr6:39400232-39400404
Coord C2 exon
chr6:39400178-39400231
Length
173 bp
Sequences
Splice sites
5' ss Seq
GTGGTAAGA
5' ss Score
8.24
3' ss Seq
GTTGTTTTTCCATGACATAGGCT
3' ss Score
5.21
Exon sequences
Seq C1 exon
GTTGTGGTGAACGCTTTGGTGGGTGCCATTCCCTCCATCATGAATGTGCTGTTGGTGTGTTTGATCTTTTGGCTGATCTTCAGTATTATGGGTGTCAACCTGTTTGCTGGAAAGTACTATTACTGCTTCAACCAGACTGCAGAGGAGTATTTTCAAGTCGACGTTGTCAATAACAAGACCGAATGCTTTGCACTCATCAATGCAAACTACACAGAAGTTCGCTGGAAGAACGTAAAGATCAATTTCGATAATGTGGGCGCAGGTTACCTGGCACTCTTGCAAGTG
Seq A exon
GTAAGACATTGTCCCTGTGTAAAAATTCTCTGAATACCCACTAACATCCCAGTTCTTAATGCTTGATGAGTAAACCAGTCTCTTCATGAATTCACAAATTATAAACACAAACATACAATTAAATATTAATGACTACCACTGTGGATTGAGCTAGTTGTTTTTCCATGACATAG
Seq C2 exon
GCTACATTCAAAGGCTGGATGGACATCATGTATGCTGCTGTGGATTCCAGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000018032:ENSDART00000151299:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0052026=Ion_trans=FE(40.9=100)
A:
NA
C2:
PF0052026=Ion_trans=FE(7.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGTCAACCTGTTTGCTGGA
R:
TTTCTGGAATCCACAGCAGCA
Band lengths:
248-421
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]