DreINT0173886 @ danRer10
Intron Retention
Gene
ENSDARG00000079564 | vmhc
Description
ventricular myosin heavy chain [Source:ZFIN;Acc:ZDB-GENE-991123-5]
Coordinates
chr2:24606182-24606789:-
Coord C1 exon
chr2:24606400-24606789
Coord A exon
chr2:24606309-24606399
Coord C2 exon
chr2:24606182-24606308
Length
91 bp
Sequences
Splice sites
5' ss Seq
AAGGTATTG
5' ss Score
7.76
3' ss Seq
AAACTGTTTACCTTGTACAGGCA
3' ss Score
5.34
Exon sequences
Seq C1 exon
GCACGTATTGAGGAGCTGGAGGAAGAGCTTGAGGCAGAAAGAGCTGCTAGAGCCAAAGTGGAGAAACAGAGAGCAGATTTAGCCAGAGAGCTGGAGGAGATCAGCGAGAGACTGGAGGAGGCTGGAGGAGCTACAGCTGCTCAGATTGAGATGAATAAGAAACGAGAGGCAGAGTTTCAGAAGCTCCGCAGAGACCTTGAAGAGGCCACTCTGCAGCATGAGGCCACTGCCGCCACACTCAGGAAAAAACAAGCCGACAGTGTGGCTGAACTTGGAGAGCAGATAGACAATCTGCAGAGGGTCAAGCAAAAACTGGAGAAGGAGAAAAGTGAACTTAGGCTGGAGTTGGATGATGTAGTCTCAAACATGGAACATGTTGTAAAGACAAAG
Seq A exon
GTATTGTAAATACAAACACTCATAAAGAAAAATGAATGTTGCTTTTCTCAGCTAAAAAGTAATTCTTAGCCAAACTGTTTACCTTGTACAG
Seq C2 exon
GCAAATCTTGAGAAGATGACCAGATCTTTAGAAGACCAAATGAATGAATATAAAACAAAATATGAGGAAGGTCAGCGCTGCATTAATGACTTCACAATGCAGAAATCTAAACTACAATCTGAAAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079564:ENSDART00000099532:26
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.438 A=NA C2=0.767
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(15.0=100),PF0003816=Filament=PU(1.8=1.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.9=100),PF0003816=Filament=FE(36.8=100),PF059116=DUF869=PU(38.6=90.7),PF0003816=Filament=PU(3.0=23.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAATCTGCAGAGGGTCAAGCA
R:
CAGCGCTGACCTTCCTCATAT
Band lengths:
183-274
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]