MmuEX0011718 @ mm9
Exon Skipping
Gene
ENSMUSG00000068917 | Clk2
Description
CDC-like kinase 2 [Source:MGI Symbol;Acc:MGI:1098669]
Coordinates
chr3:88972617-88974037:+
Coord C1 exon
chr3:88972617-88972845
Coord A exon
chr3:88973537-88973624
Coord C2 exon
chr3:88973971-88974037
Length
88 bp
Sequences
Splice sites
3' ss Seq
CCCCCCCCACCCCCCGACAGCAG
3' ss Score
6.61
5' ss Seq
GATGTACAA
5' ss Score
-5.41
Exon sequences
Seq C1 exon
CAGCTATGATGACCATTCGTCCGATCGGCGGCTGTACGATCGGCGGTACTGTGGCAGCTACAGGCGCAATGACTACAGCCGGGACAGAGGGGAGGCTTACTACGACACAGACTTTCGGCAGTCCTATGAATACCATCGAGAGAACAGCAGTTACCGAAGCCAGCGCAGCAGCCGAAGGAAACACAGAAGGCGGAGGAGACGGAGCCGGACATTCAGCCGCTCATCTTCA
Seq A exon
CAGCACAGCAGCCGGAGAGCCAAGAGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAGCGAT
Seq C2 exon
ATGAAATTGTAAGCACCTTAGGAGAAGGGACCTTCGGCCGAGTTGTGCAGTGTGTGGACCATCGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000068917_CASSETTE2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
Show structural model
Features
Disorder rate (Iupred):
C1=0.684 A=0.305 C2=0.032
Domain overlap (PFAM):
C1:
NO
A:
PF0006920=Pkinase=PU(0.1=0.0)
C2:
PF0006920=Pkinase=PU(25.0=95.7)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
ACACAGACTTTCGGCAGTCCT
R:
TCCACACACTGCACAACTCG
Band lengths:
183-271
Functional annotations
There are 1 annotated functions for this event
PMID: 16371011
Skipping of exon 4 (HsaEX0015698) increases in brain areas affected by Alzheimer's Disease resulting in increased expression levels of clk2tr. Increased expression of clk2 full-length induces skipping of clk2 exon 4. Increased expression of clk2tr does not promote skipping of clk2 exon 4.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types