BtaEX0009263 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000014393 | CLK2
Description
CDC-like kinase 2 [Source:HGNC Symbol;Acc:HGNC:2069]
Coordinates
chr3:15425452-15426842:+
Coord C1 exon
chr3:15425452-15425680
Coord A exon
chr3:15426335-15426422
Coord C2 exon
chr3:15426776-15426842
Length
88 bp
Sequences
Splice sites
3' ss Seq
CCCCCCCCGCCCCCCGACAGCAG
3' ss Score
6.97
5' ss Seq
GATGTACAA
5' ss Score
-5.41
Exon sequences
Seq C1 exon
CAGCTATGATGATCGTTCATCCGATCGGAGGGCGTATGACCGGCGATACTGTGGCAGCTACAGGCGCAACGACTACAGCCGAGATCGGGGAGAAGCCTACTATGACACAGACTACCGGCATTCCTATGAGTACCATCGGGAGAACAGCAGTTACCGCAGCCAGCGCAGCAGCCGTAGGAAGCACCGACGGCGGAGACGGCGCAGCCGGACATTCAGCCGTTCGTCTTCG
Seq A exon
CAGCACAGCAGCCGGAGAGCCAAGAGTGTAGAGGACGACGCTGAGGGCCACCTCATCTACCACGTCGGGGACTGGCTACAAGAGCGAT
Seq C2 exon
ATGAAATTGTAAGCACCTTGGGAGAGGGGACCTTCGGCCGAGTTGTACAGTGTGTTGACCATCGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000014393_CASSETTE1
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.909 A=0.568 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0006920=Pkinase=PU(0.1=0.0)
C2:
PF0006920=Pkinase=PU(6.9=95.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
AGTACCATCGGGAGAACAGCA
R:
CTGCGATGGTCAACACACTGT
Band lengths:
168-256
Functional annotations
There are 1 annotated functions for this event
PMID: 16371011
Skipping of exon 4 (HsaEX0015698) increases in brain areas affected by Alzheimer's Disease resulting in increased expression levels of clk2tr. Increased expression of clk2 full-length induces skipping of clk2 exon 4. Increased expression of clk2tr does not promote skipping of clk2 exon 4.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development