MmuEX0028544 @ mm10
Exon Skipping
Gene
ENSMUSG00000026799 | Med27
Description
mediator complex subunit 27 [Source:MGI Symbol;Acc:MGI:1916225]
Coordinates
chr2:29349846-29471386:+
Coord C1 exon
chr2:29349846-29349990
Coord A exon
chr2:29413388-29413518
Coord C2 exon
chr2:29471293-29471386
Length
131 bp
Sequences
Splice sites
3' ss Seq
TCTTCCTCTTGTCTCTGCAGCTG
3' ss Score
12.35
5' ss Seq
TCAGTGAGT
5' ss Score
7.68
Exon sequences
Seq C1 exon
TGAACTGGAACGTTTGAGCAACCTGGTAGGCAAGCCGTCTGAGAACCACCCTCTTCATAACAGTGGGCTGCTAAGCCTGGATCCTGTGCAGGACAAAACCCCACTCTACAGCCAGCTTCTGCAAGCATACAAATGGTCCAACAAG
Seq A exon
CTGCAGTACCATGCAGGTCTAGCGTCTGGCCTTTTGAACCAACAGTCCTTGAAGCGCTCTGCAAATCAGATGGGTGTGTCTGCCAAGCGGAGACCGAAGGCCCAGCCCACCACTCTGGTTCTACCACCTCA
Seq C2 exon
GTATGTCGATGACGTGATCAGCCGCATTGACAGAATGTTTCCAGAGATGTCCATTCACTTATCGAGACCCAATGGGACTTCAGCAATGCTTCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026799-'5-15,'5-14,18-15
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.293 A=0.366 C2=0.004
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAACGTTTGAGCAACCTGG
R:
CAGAAGCATTGCTGAAGTCCCA
Band lengths:
234-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types