RnoEX0053707 @ rn6
Exon Skipping
Gene
ENSRNOG00000013933 | Med27
Description
mediator complex subunit 27 [Source:RGD Symbol;Acc:1564993]
Coordinates
chr3:7887534-8032928:+
Coord C1 exon
chr3:7887534-7887678
Coord A exon
chr3:7946534-7946664
Coord C2 exon
chr3:8032821-8032928
Length
131 bp
Sequences
Splice sites
3' ss Seq
GTTCTCTCTTGTCTCTGCAGCTG
3' ss Score
11.81
5' ss Seq
TCAGTGAGT
5' ss Score
7.68
Exon sequences
Seq C1 exon
CGAACTGGAACGTTTGAGCAACCTGGTAGGCAAGCCGTCTGAGAACCACCCTCTCCATAACAGTGGGCTGCTAAGCCTGGATCCTGTGCAGGACAAAACTCCACTCTACAGCCAGCTTCTTCAAGCATACAAATGGTCCAACAAG
Seq A exon
CTGCAGTACCATGCAGGTCTAGCGTCTGGCCTTCTGAATCAACAGTCCTTGAAGCGCTCTGCAAATCAGATGGGTGTGTCTGCTAAGCGGAGACCTAAGGCCCAGCCCACCACTCTGGTTCTACCGCCTCA
Seq C2 exon
GTGACCCTGGGGAAGGTGTTGAAAGTGATCGTGGTCATGAGGAGCCTGTTCATCGACCGCACGATCGTGAAGGGGTACAACGAAAGCGTCTACACAGAGGACGGAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000013933_MULTIEX1-3/4=1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.293 A=0.305 C2=0.000
Domain overlap (PFAM):
C1:
PF091265=NaeI=PD(1.6=2.0)
A:
NO
C2:
PF115713=Med27=PU(3.4=8.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAACGTTTGAGCAACCTGG
R:
TTCCGTCCTCTGTGTAGACGC
Band lengths:
246-377
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]