MmuEX0028544 @ mm9
Exon Skipping
Gene
ENSMUSG00000026799 | Med27
Description
mediator complex subunit 27 [Source:MGI Symbol;Acc:MGI:1916225]
Coordinates
chr2:29205366-29326906:+
Coord C1 exon
chr2:29205366-29205510
Coord A exon
chr2:29268908-29269038
Coord C2 exon
chr2:29326813-29326906
Length
131 bp
Sequences
Splice sites
3' ss Seq
TCTTCCTCTTGTCTCTGCAGCTG
3' ss Score
12.35
5' ss Seq
TCAGTGAGT
5' ss Score
7.68
Exon sequences
Seq C1 exon
TGAACTGGAACGTTTGAGCAACCTGGTAGGCAAGCCGTCTGAGAACCACCCTCTTCATAACAGTGGGCTGCTAAGCCTGGATCCTGTGCAGGACAAAACCCCACTCTACAGCCAGCTTCTGCAAGCATACAAATGGTCCAACAAG
Seq A exon
CTGCAGTACCATGCAGGTCTAGCGTCTGGCCTTTTGAACCAACAGTCCTTGAAGCGCTCTGCAAATCAGATGGGTGTGTCTGCCAAGCGGAGACCGAAGGCCCAGCCCACCACTCTGGTTCTACCACCTCA
Seq C2 exon
GTATGTCGATGACGTGATCAGCCGCATTGACAGAATGTTTCCAGAGATGTCCATTCACTTATCGAGACCCAATGGGACTTCAGCAATGCTTCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026799-'3-10,'3-9,9-10
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.327 A=0.366 C2=0.004
Domain overlap (PFAM):
C1:
PF091265=NaeI=PD(0.1=0.0)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAACGTTTGAGCAACCTGG
R:
CAGAAGCATTGCTGAAGTCCCA
Band lengths:
234-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: