Special

MmuEX0035637 @ mm9

Exon Skipping

Gene
ENSMUSG00000087141 | Plcxd2
Description
phosphatidylinositol-specific phospholipase C, X domain containing 2 [Source:MGI Symbol;Acc:MGI:3647874]
Coordinates
chr16:45972222-46010331:-
Coord C1 exon
chr16:46009807-46010331
Coord A exon
chr16:45980349-45980809
Coord C2 exon
chr16:45972222-45972463
Length
461 bp
Sequences
Splice sites
3' ss Seq
TCCTTTATTGCTTTATCCAGGCT
3' ss Score
6.93
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
Exon sequences
Seq C1 exon
AGTAGCTCCGGGTGTGGCTGTTCCTGGATGTTGTGGGTTTCAGGAAGGTTTGTATCTTCGGGCCTGCCTCCCCCGTCTCCAGAGGGGAACTAAGAAGTTTAACTGACCAGGGACTGAGGAGATTAAAGGAGTGGATCTCAGTGGCAAGGCGAAGAGGGTGGGGACTGCGGGAGCAGCAGGCAGGGGAGCACCTATTTGACCCATCTCCCAGCTAGAAAAGGATCTTGAAAAGACTGGCGTGACCCATGTGCTCTGAAATGTTTAGAGAGCCCCGAGCGGTGATGACCACAGAGTGAGTGGCACTAGACTGAGCCTGTCTAAATCGTGGACGACAGGGATGCTTGCATTTAGAAAGGCCAGAAGGAAACTCAGGATGGGTACCATCTGCTCTCCCAATCCCAGCGGGACAAAGACAGCCTCGGAGGTCTGCAATGCCGACTGGATGGCCTCGCTCCCCGCTCACCTCCACAACGTCCCCCTTTCCAATCTGGCAATCCCAG
Seq A exon
GCTCCCATGATTCATTCAGCTACTGGGTAGATGAAAAGTCCCCAGTGGGGCCTGACCAAACCCAAGCTGTCATACGCCTTGCCCGGATCTCCTTGGTGAAAAAGTTAATGAAGAAATGGTCTGTAACTCAGAACCTGACCTTCAGGGAGCAGCTGGAGGCTGGGATCCGCTACTTTGACCTACGGGTGTCTTCCAAGCCTGGAGACACTGACCAGGAGATCTACTTCATCCACGGGCTCTTTGGCATCAAGGTTTGGGATGGGTTGATGGAGATTGATGCCTTCCTCACTCAGCACCCTCAAGAGATCATCTTCCTGGATTTCAACCACTTCTATGCCATGGATGAGAGCCACCACAAATGCCTGGTTCTGCGGATCCAGGAGGCCTTTGGGAACAAGCTGTGCCCAGCCTGCAGCGTGGAAAGCATGACACTGCGGAGTCTGTGGGAGAAAAAGTACCAG
Seq C2 exon
GTTCTCATTTTCTACCACTGTCCCTTCTACAAGCAATACCCATTCCTGTGGCCAGGGAAGAAGATTCCGGCACCCTGGGCAAACACCACGAGTGTGCAGAAACTGATCCTCTTCTTGGAGACCACCCTGAGTGAGCGAGCTCCACGTGGGGCCTTCCATGTCTCCCAGGCCATCCTCACGCCAAGAGTGAAGACTATTGCTCGAGGCCTGGTGGGGGGCCTCAAGAACACACTAGTTCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000087141-'0-1,'0-0,1-1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.145 A=0.000 C2=0.000
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF034908=Varsurf_PPLC=PU(54.3=45.5),PF0038814=PI-PLC-X=PU(5.3=16.4)

							
A:
PF034908=Varsurf_PPLC=PD(43.5=13.0),PF0038814=PI-PLC-X=FE(90.5=100)

							
C2:
PF0038814=PI-PLC-X=PD(3.0=6.2)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000114852





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr3:111707926-111708386 
HIGH PSI
HsaEX6057614
(PLCXD2)
Human
(hg19)
chr3:111426773-111427233 
HIGH PSI
HsaEX6057614
(PLCXD2)
Rat
(rn6)
chr11:57238832-57239292 
HIGH PSI
RnoEX0066825
(Plcxd2)
Cow
(bosTau6)
chr1:56878513-56878973 
ALTERNATIVE
BtaEX0027473
(PLCXD2)
Chicken
(galGal4)
chr1:87804315-87804775 
Chicken
(galGal3)
chr1:91708825-91709285 
GgaEX0022537
(PLCXD2)
Zebrafish
(danRer10)
chr24:25038882-25039348 
HIGH PSI
DreEX6095211
(PLCXD2)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
TTGAAAAGACTGGCGTGACCC

				
R: 
CAGGGTGCCGGAATCTTCTTC

				
Band lengths: 
353-814

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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