MmuEX6066069 @ mm10
Exon Skipping
Gene
ENSMUSG00000035200 | Chrnb4
Description
cholinergic receptor, nicotinic, beta polypeptide 4 [Source:MGI Symbol;Acc:MGI:87892]
Coordinates
chr9:55036392-55043925:-
Coord C1 exon
chr9:55043777-55043925
Coord A exon
chr9:55036587-55036631
Coord C2 exon
chr9:55036392-55036501
Length
45 bp
Sequences
Splice sites
3' ss Seq
ACCCCCCCTTCCCTCCCCAGAAT
3' ss Score
12.26
5' ss Seq
CAGGTAAGT
5' ss Score
10.86
Exon sequences
Seq C1 exon
GGGACTGCCGCCTGGCCAACGCAGAGGAGAAGCTGATGGATGATCTCCTGAACAAAACCCGCTACAACAACCTGATCCGCCCAGCCACCAGCTCCTCCCAGCTCATCTCCATCCGCCTGGAGCTATCACTGTCCCAGCTCATCAGTGTG
Seq A exon
AATGAGCGAGAACAGATCATGACCACCAGCATCTGGCTGAAACAG
Seq C2 exon
GAATGGACTGACTACCGCCTGGCCTGGAACAGCTCCTGCTATGAAGGGGTGAACATTCTGAGGATTCCTGCAAAGCGAGTCTGGTTGCCTGACATCGTGTTGTACAACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035200-'4-7,'4-6,7-7
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=PU(19.9=82.0)
A:
PF0293118=Neur_chan_LBD=FE(6.8=100)
C2:
PF0293118=Neur_chan_LBD=FE(17.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGGAGCTATCACTGTCCCA
R:
AACACGATGTCAGGCAACCAG
Band lengths:
135-180
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types