Special

MmuEX6068667 @ mm10

Exon Skipping

Gene
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B [Source:MGI Symbol;Acc:MGI:107561]
Coordinates
chr9:107599803-107600371:-
Coord C1 exon
chr9:107600330-107600371
Coord A exon
chr9:107599950-107600107
Coord C2 exon
chr9:107599803-107599858
Length
158 bp
Sequences
Splice sites
3' ss Seq
GAGACTCTTGTGCCCTCTAGCAA
3' ss Score
4.56
5' ss Seq
GAGGTGGGT
5' ss Score
7.07
Exon sequences
Seq C1 exon
GACTCTGCCGCTATCACCAGCATGCAAATCTCCTCTAAAAGG
Seq A exon
CAACAACTCTACATAGCATCGCGCAGCGCAGTGGCCCAGATTGCTTTGCATCGCTGCACTGCCCTAGGCCGCGCCTGCGCAGAATGCTGCTTGGCCCGTGATCCTTACTGCGCCTGGGATGGATCAGCTTGCACACGCTTCCAGCCTACGGCCAAGAG
Seq C2 exon
ACGGTTCCGGAGGCAAGACATAAGGAATGGCGACCCCAGCACCCTATGCTCTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057969_MULTIEX1-3/3=1-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact

ORF disruption upon sequence exclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.010 A=0.009 C2=0.291
Domain overlap (PFAM):

C1:
PF0140314=Sema=FE(2.9=100)
A:
NO
C2:
PF0143720=PSI=FE(29.7=100),PF0768612=V-set=PU(0.1=0.0)


Main Inclusion Isoform:


Main Skipping Isoform:
NA


Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
HIGH PSI
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACTCTGCCGCTATCACCAG
R:
CATAGGGTGCTGGGGTCGC
Band lengths:
90-248
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types