MmuEX6100075 @ mm9
Exon Skipping
Gene
ENSMUSG00000039844 | Rapgef1
Description
Rap guanine nucleotide exchange factor (GEF) 1 [Source:MGI Symbol;Acc:MGI:104580]
Coordinates
chr2:29589097-29590039:+
Coord C1 exon
chr2:29589097-29589282
Coord A exon
chr2:29589720-29589804
Coord C2 exon
chr2:29589945-29590039
Length
85 bp
Sequences
Splice sites
3' ss Seq
ATGCTTATGCTGTATTCCAGGCC
3' ss Score
7.42
5' ss Seq
GAGGTATGA
5' ss Score
8.55
Exon sequences
Seq C1 exon
CCTGGTGGAGCTGACAGAGGAGATCCTGAAGCTGCTGATGGAGCTGGTCTTCCGCCTAGTGTGCAGCGGAGAGCTCAGCCTGGCCAGAGTCCTCCGGAAGAACATTCTGGACAAGGTGGACCAGAAGAAGCTGCTCAGGTGTGCCCATTCCGACCAGCCTCTGGCAGCCAGGGGTGTTGCAGCCAG
Seq A exon
GCCAGGAACCTTGCATGATTTCCACAGCCACGAGATAGCTGAGCAGCTGACACTGCTGGATGCCGAGCTTTTCTACAAGATAGAG
Seq C2 exon
ATTCCTGAAGTTTTGCTTTGGGCCAAAGAGCAGAATGAGGAGAAGAGTCCCAATCTGACCCAGTTCACAGAGCACTTCAACAACATGTCCTACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000039844-'30-35,'30-34,32-35=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.094
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PD(19.2=30.2)
A:
PF0061714=RasGEF=PU(12.9=79.3)
C2:
PF0061714=RasGEF=FE(17.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGAGTCCTCCGGAAGAACA
R:
TGCTCTGTGAACTGGGTCAGA
Band lengths:
177-262
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: