DreEX0060803 @ danRer10
Exon Skipping
Gene
ENSDARG00000043593 | rapgef1a
Description
Rap guanine nucleotide exchange factor (GEF) 1a [Source:ZFIN;Acc:ZDB-GENE-081105-26]
Coordinates
chr8:11748633-11751117:-
Coord C1 exon
chr8:11750932-11751117
Coord A exon
chr8:11750771-11750855
Coord C2 exon
chr8:11748633-11748727
Length
85 bp
Sequences
Splice sites
3' ss Seq
TTGACTGTTTATTTCATCAGGCC
3' ss Score
7.27
5' ss Seq
GAGGTACAG
5' ss Score
6.62
Exon sequences
Seq C1 exon
TTTGGTGGAGCTGACGGAAGACATCCTGAAGCAGCTCATGGAGCTTGTGTTCAATCTCTTGTGTAAAGGGGAGCTTACTTTGGCCCGGGTTCTGCGCAAAAACATCCTGGATAAAGTCCAGCAGAAGAAGTTGCTGCAGTACACTAATTCCCTCAAAACACTGGCTGCACGCGGTGTGGCCGCCAG
Seq A exon
GCCTGGCACATTGCATGACTTCCGTAGCCATGAGATCGCAGACCAGCTGACGCTCCTCGATGCAGAGCTCTTTTATAAGATAGAG
Seq C2 exon
ATCCCGGAGGTTTTACTTTGGGCAAAAGAACAGAATGAGGAGAAAAGCCCCAACCTGACCCAATTCACAGAGCACTTCAACAACATGTCGTACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000043593-'18-24,'18-23,19-24
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.091
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PD(29.2=52.4)
A:
PF0061714=RasGEF=PU(12.9=79.3)
C2:
PF0061714=RasGEF=FE(18.0=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGTTCTGCGCAAAAACATCC
R:
GTGAATTGGGTCAGGTTGGGG
Band lengths:
168-253
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]