BtaEX6046942 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020791 | RAPGEF1
Description
Rap guanine nucleotide exchange factor (GEF) 1 [Source:HGNC Symbol;Acc:HGNC:4568]
Coordinates
chr11:101732453-101733518:-
Coord C1 exon
chr11:101733333-101733518
Coord A exon
chr11:101732690-101732774
Coord C2 exon
chr11:101732453-101732547
Length
85 bp
Sequences
Splice sites
3' ss Seq
ATAGTGCCTCTGTCTTCCAGGCC
3' ss Score
8.65
5' ss Seq
GAGGTACAG
5' ss Score
6.62
Exon sequences
Seq C1 exon
CCTGGTAGAGCTGACAGAAGAGATCCTGAAGCTGCTGATGGAGCTGGTCTTCCGCCTGGTGCGCAGCGGGGAGCTCAGCCTAGCACGCGTGCTCCGCAAGAACATTCTGGACAAGGTCGACCAGAAGAAGCTGCTCCGGTGCGCCAACTCTGACCAGCCCCTGGCGGCCCGGGGAGTGGCAGCCAG
Seq A exon
GCCCGGGACCTTGCACGACTTCCACAGCCATGAAATAGCCGAGCAGCTGACTCTGCTAGATGCCGAGCTCTTTTATAAAATAGAG
Seq C2 exon
ATTCCTGAGGTTTTGCTTTGGGCTAAAGAGCAGAACGAGGAGAAGAGCCCGAACCTGACCCAGTTCACGGAGCACTTCAACAACATGTCCTACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020791-'30-45,'30-44,31-45=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.094
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PD(21.6=34.9)
A:
PF0061714=RasGEF=PU(12.9=79.3)
C2:
PF0061714=RasGEF=FE(17.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTCCGCAAGAACATTCTGGA
R:
TTGAAGTGCTCCGTGAACTGG
Band lengths:
176-261
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]