MmuINT0040594 @ mm9
Intron Retention
Gene
ENSMUSG00000025650 | Col7a1
Description
collagen, type VII, alpha 1 [Source:MGI Symbol;Acc:MGI:88462]
Coordinates
chr9:108858407-108858752:+
Coord C1 exon
chr9:108858407-108858523
Coord A exon
chr9:108858524-108858605
Coord C2 exon
chr9:108858606-108858752
Length
82 bp
Sequences
Splice sites
5' ss Seq
GTGGTAAGT
5' ss Score
10.36
3' ss Seq
CTCTGTGTACTCGCATACAGGTG
3' ss Score
9.17
Exon sequences
Seq C1 exon
CTGCCAAGGAAGGGCTAGAATTGAGCCTCCAGAACATCACATCGCACAGCCTCCTGGTGGCCTGGAGGAGGGTACCAGGTGCTAATGGCTACCGTGTAACATGGCGGGACCTCAGTG
Seq A exon
GTAAGTGAGAGGCATGGACTGACAAAGTTATCGATTCTTACATCTATAAGGCCCACTGAACCCTCTGTGTACTCGCATACAG
Seq C2 exon
GTGGGCCAACCCAGCAGCAGGACCTGAGCCCTGGACAGGGGTCAGTGTTTCTAGATCACTTGGAGCCTGGCACGGACTATGAGGTGACCGTGAGCGCCCTATTTGGCCACAGCGTGGGGCCTGCCGCTTCTCTGACTGCCCGCACCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025650-Col7a1:NM_007738:8
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.300 A=NA C2=0.420
Domain overlap (PFAM):
C1:
PF0004116=fn3=PU(44.2=85.0)
A:
NA
C2:
PF0004116=fn3=PD(54.5=84.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGGCTAGAATTGAGCCTCCA
R:
TGCCAGGCTCCAAGTGATCTA
Band lengths:
179-261
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: