MmuINT0087290 @ mm10
Intron Retention
Gene
ENSMUSG00000074657 | Kif5a
Description
kinesin family member 5A [Source:MGI Symbol;Acc:MGI:109564]
Coordinates
chr10:127236054-127236830:-
Coord C1 exon
chr10:127236721-127236830
Coord A exon
chr10:127236156-127236720
Coord C2 exon
chr10:127236054-127236155
Length
565 bp
Sequences
Splice sites
5' ss Seq
AGAGTAAGG
5' ss Score
5.42
3' ss Seq
TTATGTCCCACCCTCCCCAGCCT
3' ss Score
6.41
Exon sequences
Seq C1 exon
AAGGCCCTGGAACTACAGATGGAGAATCACCGTGAGGCCCATCACCGGCAGCTGGCCCGCCTCCGGGATGAGATTAATGAGAAACAGAAAACCATTGATGAGCTGAAAGA
Seq A exon
GTAAGGGGCGGGGCTTAGAGGTGGCTCCAGTGTGTCTGTCAGTCCCCTCATCTTGTTGTGGCCACTCCAGCGATCCTTCTGGATCTTTAATGTCCTCAACAGTGACACGCCTTGAGGCCCAAACTTGAGAGACACGAGAAGGGCTGGAGAGGTGGCTCTGTAGTTTGAGAGCACTGGCTTGCTTTTCCAGAGAACCTGAGTTTGATTCCTAGCAACCACGTGGTGGCTCGCAACCGTCTGTAACTCCAGTCCCAGGGGATCTGACTGCCTCTTCTGGCCTCCTTGGGTACCAGGCACACAGACATACTCGCAAGCAAAACACCCATACACATAAAAAATTAATTAAAAGACACAAGTAGTTAGGATTAGACCTGTCCTTGGAACTTTTATGATTGCAGTACTCGGGGTCCCATGTTGCCATGAAAGTGGTAGCCGACGTGTATCCCCGATAGAGCCAGTGTGTATCCCTGATATCTGAAGTCGGGTGCATCAGGGCTGGTGTGAGGGGCGGGGCTTCCTCCCCCCCCCCCCCATTGAGTGTCTCTTTATGTCCCACCCTCCCCAG
Seq C2 exon
CCTGAACCAGAAGCTCCAGTTAGAGCTGGAGAAGCTTCAGGCCGACTATGAGAGGCTGAAGAATGAAGAGAACGAGAAGAGCGCCAAGCTCCAGGAGCTGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000074657:ENSMUST00000099172:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.784 A=NA C2=0.429
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGGCCCTGGAACTACAGATG
R:
CTTGGCGCTCTTCTCGTTCTC
Band lengths:
198-763
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types