MmuINT0170583 @ mm9
Intron Retention
Gene
ENSMUSG00000020099 | Unc5b
Description
unc-5 homolog B (C. elegans) [Source:MGI Symbol;Acc:MGI:894703]
Coordinates
chr10:60236492-60237490:-
Coord C1 exon
chr10:60237101-60237490
Coord A exon
chr10:60236580-60237100
Coord C2 exon
chr10:60236492-60236579
Length
521 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGC
5' ss Score
9.88
3' ss Seq
TAGCCTTCCTTCCTCCCTAGGGG
3' ss Score
10.25
Exon sequences
Seq C1 exon
ACAACCCGCAGCTCCTGCACCCGTCCGCCCCTCCAGACCTAACGGCCAGTGCTGGCATCTACCGCGGGCCTGTGTATGCCCTGCAGGACTCCGCCGACAAGATCCCCATGACTAATTCGCCCCTGCTGGATCCCCTGCCCAGCCTCAAGATCAAGGTCTATAACTCCAGCACCATCGGTTCTGGGTCTGGCCTGGCTGATGGAGCCGACCTGCTGGGTGTCCTCCCGCCGGGCACGTACCCAGGCGATTTCTCCCGGGACACCCATTTCCTGCACCTGCGCAGTGCCAGCCTTGGTTCCCAGCACCTCCTGGGCCTACCTCGGGACCCCAGCAGCAGTGTCAGCGGCACCTTTGGTTGCCTGGGAGGAAGGCTGAGCCTCCCCGGCACAG
Seq A exon
GTAGGCCTCCAGTTCCATTGCTCAGGTGTGAAGTTATGGCTTGGAGCAGATTACCCATAGAGAAGTCTGGTAGAAGACCTGAGATCAGGACTTCCTGGGCCTCTGTAGGCCAGGAAAGTCTTGGGGCAACCACTTGGAAGGGGTCTCACTTCCCTTTAGGTTGGGAGAATGGCTCTTATCTTTAGTCCCATAGAGCTGTCCTGGTTTTAAAGGGCAGCAGGGCCAGGATGCGCATCTCAGCTTGTGAAAGTGGCAGCTGGTGGCTTTTCAGAGTCCTGTCCTGAGCCAGCATGAGGGCTAGCACTGGAGCATTCTGACCCTCCTACCCCAGCAATGTTAGAGGCCCCCTGTATTCATGGGCAGACCAGGCCAAGTAGCCTGTGTGCTCCCTGTATCTATCCACCCCCATACCCCCGAGTCTAGAGTCTTTCCCTTCCCTAGGTTGGAGCCAGAGTCTACCCCATCTTTCTCATCTCTGCTACCTCCTCCTCCTCTTGCTGATAGCCTTCCTTCCTCCCTAG
Seq C2 exon
GGGTCAGCCTGTTGGTACCAAATGGAGCCATTCCCCAGGGCAAGTTCTATGACCTGTATCTACATATCAACAAGGCCGAAAGCACCCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020099-Unc5b:NM_029770:10
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.076 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0079115=ZU5=PU(20.4=16.0)
A:
NA
C2:
PF0079115=ZU5=FE(28.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCCCAGCCTCAAGATCAAG
R:
GGTGCTTTCGGCCTTGTTGAT
Band lengths:
342-863
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: