RnoEX0037765 @ rn6
Exon Skipping
Gene
ENSRNOG00000057044 | Garnl3
Description
GTPase activating Rap/RanGAP domain-like 3 [Source:RGD Symbol;Acc:1564019]
Coordinates
chr3:12115159-12155098:-
Coord C1 exon
chr3:12154956-12155098
Coord A exon
chr3:12136894-12136964
Coord C2 exon
chr3:12115159-12115233
Length
71 bp
Sequences
Splice sites
3' ss Seq
TGAAACCATTTTTGTTCCAGCTC
3' ss Score
7.47
5' ss Seq
CTGGTAAGT
5' ss Score
10.65
Exon sequences
Seq C1 exon
ATGGATCCACTGACCAAGGGGTCCTGTGGGAGTCAGCTGGCACAAACCCTCCTCTGGAAAGCGAAGTCATCTCTCTCTTTCGGCATCCAGCCCCTTCAGACTTGGCCAACAAAGGATCCTGAACTAGAGTCCCAGGTCAACCT
Seq A exon
CTCTGTGCTCAGAAGACCTAGGCTGTAGACGTGGGGATTTCAGTAGGAAACATTATGGATCTGTGGAGCTG
Seq C2 exon
CTCATCTCTAGTGATGCAGATGGAGCCATCCAAAGAGCAGGAAGATTCCGAGTGGAAAATGGCTCTACAGATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000057044_MULTIEX2-1/2=C1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.028 A=0.000 C2=0.320
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCAGCTGGCACAAACCCTC
R:
TCCACTCGGAATCTTCCTGCT
Band lengths:
168-239
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with negative fitness/survivability impact when depleted: HeLa Depleted at 14 days (FC=0.774, FDR=0.001), PC9 No Change at 14 days (FC=1.238, FDR=0.436), Late Xenograft No change (FC=0.852, FDR=0.023).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]