RnoEX6016240 @ rn6
Exon Skipping
Gene
ENSRNOG00000015396 | Sptan1
Description
spectrin, alpha, non-erythrocytic 1 [Source:RGD Symbol;Acc:621714]
Coordinates
chr3:8573540-8575472:+
Coord C1 exon
chr3:8573540-8573563
Coord A exon
chr3:8574275-8574310
Coord C2 exon
chr3:8575425-8575472
Length
36 bp
Sequences
Splice sites
3' ss Seq
GCTCCTTTTTCTTTGACTAGGAT
3' ss Score
8.84
5' ss Seq
AAGGTAAGG
5' ss Score
10.51
Exon sequences
Seq C1 exon
GAGGTGTATGGTATGATGCCCAGG
Seq A exon
GATGAAGCAGATTCCAAGACCGCCTCCCCATGGAAG
Seq C2 exon
TCTGCTCGACTGATGGTCCACACAGTGGCCACCTTCAACTCCATCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000015396-'49-53,'49-52,50-53=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.903 A=0.927 C2=0.030
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(2.4=25.0)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGGTGTATGGTATGATGCCCA
R:
CTTGATGGAGTTGAAGGTGGCC
Band lengths:
72-108
Functional annotations
There are 1 annotated functions for this event
PMID: 9712874
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: classical fluorescence spectroscopy, cleavage reaction, glutathione s tranferase tag, western blot. ELM ID: ELMI001909; ELM sequence: DETDS; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]