BtaEX6046934 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020791 | RAPGEF1
Description
Rap guanine nucleotide exchange factor (GEF) 1 [Source:HGNC Symbol;Acc:HGNC:4568]
Coordinates
chr11:101767464-101768781:-
Coord C1 exon
chr11:101768629-101768781
Coord A exon
chr11:101768056-101768179
Coord C2 exon
chr11:101767464-101767650
Length
124 bp
Sequences
Splice sites
3' ss Seq
GGCTCCTTGCTCTGTTTCAGTCC
3' ss Score
7.99
5' ss Seq
CAGGTAATT
5' ss Score
8.55
Exon sequences
Seq C1 exon
CCAGTCTGAGCTCCCCCTGACGGATCGAGAGATGGAGATACTAAACAAGACGACCAGCCTGTCACAGTCAACCGAGGGGCTCCAAGACTCTATGGACGAAGAGGTTGCACCCCCCAAGCCCCCCTTACCTGGCATTAGGGTGGTTGATAACAG
Seq A exon
TCCTCCACCAGCACTGCCCCCGAAGAAGAGACAGTCGGCTCCGTCCCCCACGCGTGTGGCCGTTGTGGCCCCCATGAGTCGAGCCACCAGTGGCTCCAGTTTGCCTGTTGGAATTAATAGGCAG
Seq C2 exon
GAGGACTTTGACGTGGACTGTTACGCACAGAGACGGCTGTCGGGAGGCAGCCACTCGTACAGCGGTGAGTCTCCCCGCCTGTCGCCCTGCAGCAGCATCGGCAAGCTCAGCAAGTCGGACGAGCAGCTGTCCTCTCTGGACAGGGACAGCGGGCAGTGCTCCCGGAACACAAGCTGTGAAACACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020791-'14-23,'14-21,15-23=AN
Average complexity
A_C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.952 C2=0.810
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCAGCCTGTCACAGTCAAC
R:
TCCAGAGAGGACAGCTGCTC
Band lengths:
242-366
Functional annotations
There are 1 annotated functions for this event
PMID: 7735837
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: x-ray crystallography. ELM ID: ELMI001098; ELM sequence: PALPPK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]