MmuEX6100084 @ mm9
Exon Skipping
Gene
ENSMUSG00000039844 | Rapgef1
Description
Rap guanine nucleotide exchange factor (GEF) 1 [Source:MGI Symbol;Acc:MGI:104580]
Coordinates
chr2:29555206-29556371:+
Coord C1 exon
chr2:29555206-29555358
Coord A exon
chr2:29555706-29555826
Coord C2 exon
chr2:29556188-29556371
Length
121 bp
Sequences
Splice sites
3' ss Seq
GGATTTTCTGTCTCTTCTAGTCC
3' ss Score
9.3
5' ss Seq
CAGGTAATG
5' ss Score
9.43
Exon sequences
Seq C1 exon
CCAGCCTGAGCTCCCTCTGACAGACCGAGAAATGGAGATTCTGAACAAGACGACAAGTGTGTCACCATCTGCTGAACTGCTCCCAGACTCCACCAGTGAAGAGGTCGCACCCCCCAAGCCCCCTTTACCTGGCATCCGGGTGGTTGATAACAG
Seq A exon
TCCACCAGCATTACCACCCAAGAAAAGGCAGTCTGCTCCATCCCCCACTCGGGTGGCTGTGGTAGCCCCAATGAGTCGGGCTACCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAG
Seq C2 exon
GACTTTGATGTTGAATGTTACACCCAGAGGCGCCTGTCAGGAGGCAGCCGCTCCTGCGGTGGTGAGTCTCCTCGCCTGTCCCCCTGCAGCAGCACAGGCAAGCTCAGCCGCTCAGACGAGCAGCTGTCCTCCCTGGACAGGGATAGTGGGCAGTGCTCACGGAACACAAGCTGTGAAACACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000039844-'13-13,'13-12,15-13=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.985 A=0.941 C2=0.774
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATCTGCTGAACTGCTCCCAG
R:
ACTGCCCACTATCCCTGTCC
Band lengths:
242-363
Functional annotations
There are 1 annotated functions for this event
PMID: 7735837
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: x-ray crystallography. ELM ID: ELMI001098; ELM sequence: PALPPK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: