DreEX0060806 @ danRer10
Exon Skipping
Gene
ENSDARG00000043593 | rapgef1a
Description
Rap guanine nucleotide exchange factor (GEF) 1a [Source:ZFIN;Acc:ZDB-GENE-081105-26]
Coordinates
chr8:11782011-11793637:-
Coord C1 exon
chr8:11793482-11793637
Coord A exon
chr8:11785376-11785496
Coord C2 exon
chr8:11782011-11782191
Length
121 bp
Sequences
Splice sites
3' ss Seq
CACAAACACTCTCTTCGCAGTCC
3' ss Score
7.47
5' ss Seq
CAGGTGAGA
5' ss Score
9.22
Exon sequences
Seq C1 exon
TGTCCCTGAGCCCCCTGTCATTGAGACGAAGGAACCCATCTTGAATGACAGCCCTGCCCCAGCTGAGAACATTGAGACCCCAAATGATGTACCTGAGGAGGAAGTAGCCCCTCCGAAACCCCCTCTACCTGGACTCAAAGTGCCTGAACAACACTG
Seq A exon
TCCACCGGCTCTCCCTCCAAAGAAGCGTCAGTCGGCACCGTCTCCCACCAGGATTGCTGTGGTTGCGCCAATGAGTCGCACCACAAGTGGATCAAGTCTTCCCATTGGTATCTGTCGACAG
Seq C2 exon
GAGTATGAAGCAGATGCCTTACAAAGGCGTTTTTCTGGAGGCAGTCACTCATATGGAGGAGAATCGCCACGACTGTCCCCATGTGGCAGCATCGGCATATTGAGCAAGTCAGATGAACAGCTGTCCTCTCTGGATCAGGACAGTGGACAGTGCTCTCGTAACACCAGCTGTGAGACTCTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000043593-'5-9,'5-8,6-9
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.951 C2=0.885
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCCCAGCTGAGAACATTGAG
R:
AGCACTGTCCACTGTCCTGAT
Band lengths:
255-376
Functional annotations
There are 1 annotated functions for this event
PMID: 7735837
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: x-ray crystallography. ELM ID: ELMI001098; ELM sequence: PALPPK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]