RnoEX0073984 @ rn6
Exon Skipping
Gene
ENSRNOG00000014316 | Rapgef1
Description
Rap guanine nucleotide exchange factor 1 [Source:RGD Symbol;Acc:619793]
Coordinates
chr3:8254635-8255888:+
Coord C1 exon
chr3:8254635-8254784
Coord A exon
chr3:8255198-8255318
Coord C2 exon
chr3:8255705-8255888
Length
121 bp
Sequences
Splice sites
3' ss Seq
GGATTTTCTGTCTCTTCTAGTCC
3' ss Score
9.3
5' ss Seq
CAGGTAATG
5' ss Score
9.43
Exon sequences
Seq C1 exon
CCAGCCTGAGCTCCCTCTGACAGAACGAGAAATGGAGATTCTGAACAAGACAAGTGTGTCACCATCCACTGAACTGCTCCCAGACTCCACCAGTGAAGAGGTTGCGCCCCCCAAGCCCCCTTTACCTGGCATCCGGGTGGTTGATAACAG
Seq A exon
TCCACCAGCATTACCACCCAAGAAAAGGCAATCTGCTCCATCCCCTACCCGGGTGGCTGTGGTAGCCCCCATGAGTCGTGCTGTCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAG
Seq C2 exon
GACTTTGATGTTGAATGTTACACCCAGAGGCGCCTGTCAGGAGGCAGCCGCTCCTGTGGTGGTGAATCTCCTCGCCTGTCCCCCTGCAGCAGTACAGGCAAGCTCAGCCGGTCAGACGAGCAGCTGTCCTCCCTAGACAGGGATAGCGGGCAGTGCTCACGGAACACAAGCTGTGAAACACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014316-'23-21,'23-19,26-21
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=0.951 C2=0.839
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCTGAGCTCCCTCTGACAGAA
R:
CTGAGCTTGCCTGTACTGCTG
Band lengths:
253-374
Functional annotations
There are 1 annotated functions for this event
PMID: 7735837
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: x-ray crystallography. ELM ID: ELMI001098; ELM sequence: PALPPK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]