DmeEX6010542 @ dm6
Exon Skipping
Gene
FBgn0086757 | cbs
Description
The gene centrosomin's beautiful sister is referred to in FlyBase by the symbol Dmelcbs (CG4840, FBgn0086757). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (3 unique). Gene sequence location is 2R:13450586..13453393. Its molecular function is described by: ADP-ribosylation factor binding; Rab GTPase binding. It is involved in the biological process described with: centrosome cycle; Golgi vesicle transport. 8 alleles are reported. The phenotypes of these alleles manifest in: spindle; centrosome; embryo; nucleus. The phenotypic classes of alleles include: viable; mitotic cell cycle defective; lethal - all die before end of embryonic stage; some die during embryonic stage. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
Coordinates
chr2R:13451090-13452735:+
Coord C1 exon
chr2R:13451090-13451875
Coord A exon
chr2R:13451928-13452026
Coord C2 exon
chr2R:13452322-13452735
Length
99 bp
Sequences
Splice sites
3' ss Seq
CTTATGCACTTTAATTGCAGGTC
3' ss Score
8.06
5' ss Seq
TCTGTGAGT
5' ss Score
7
Exon sequences
Seq C1 exon
CCCAGCAAATCGAGTCCTTGAGCGAGGAACTAGAACGTCTGCGTCCCATTGAATCCGTGGCCGAAGATTTACGCGACGAACTAGAGCAACTGCGACATTCTACACAGCAGGAGAAGAATCTGCTGACCACAACACTGGCGGCTGTACAGGAGGAGAACCGGCACCTCAAAAAGCGCATGAAAATCGTCGAGGAGTCGCGACTCGAGTCTCTGGGCAAACTGAACTCCGAGCAACAAGTGCAAGCCCTTATCCGGGAACATAAACTGCTGGAACAGCACTTAGAGGAGGCCCATCTGCAGTTGTCGGACATCAAAGGCTCGTGGAGTGGCCAGAATCTGGCGCTGGAAACCCAAGTTAGTCGTCTATCCAAGCAAGTGGCCGAAGAGACCACCGAAAAGCGAAAGGCCCTTAAATCCCGCGACGATGCCATTGAAAGCCGCAAGCAGGTATCATTCGAACTCGAAAAAGCCAAAGATGAAATAAAGCAGCGGGACGATAAG
Seq A exon
GTCAAGCTGCTGGAGGAGGAAATCGATGAACTTAGTGTGGCCCTGAAAGAGTGCCGAGAGGAGAACGAGCAGCAAGTCCTCTTTGAGCGCAATAAATCT
Seq C2 exon
CAAAATTTGGAAACAGAGGTAAAAGATCTCAAGACTCGTCTCACGGCAGCGGATGACAGGTTCAGCGAGTACTCCTCGAATGCCGAGCAGGTGGCCCAAAAGCTACGGGTTCAGGTGACTGAAAAGCAGGAGCAGCTGGACGAAACCATAATGCAACTGGAAATCGAACGAGAGGAGAAAATGACTGCAATACTTCGCAATGCGGAAATCGCCCAGAGCGAAGACATTTTACGGCAACAGCTGCGCTTGGAGCGCAGCGAAGCCAGCGACCTCCAGGAGAGGAATAATCAGCTGGTGAGGGATATATCCGAGGCACGGCAAACGTTGCAGCAGGTCAGCAGCACAGCACAGGATAATGCCGACAAGTTGACCGAATTCGAGCGAGTTCAGTTGGAAATAATCGAAAAGAATAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0086757-'1-3,'1-1,2-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.183 A=0.273 C2=0.384
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Inclusion Isoform:
FBpp0086786
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0301620, FBpp0301621
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGCAAGCAGGTATCATTCGA
R:
ACCTGAACCCGTAGCTTTTGG
Band lengths:
180-279
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)