DmeEX6023903 @ dm6
Exon Skipping
Gene
FBgn0003137 | Ppn
Description
The gene Papilin is referred to in FlyBase by the symbol DmelPpn (CG33103, FBgn0003137). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 3R:28520665..28540324. Its molecular function is described by: serine-type endopeptidase inhibitor activity; peptidase activity; extracellular matrix structural constituent. It is involved in the biological process described with: extracellular matrix organization. 9 alleles are reported. The phenotypes of these alleles manifest in: embryonic/larval tracheal system; embryonic/larval somatic muscle; Malpighian tubule. The phenotypic classes of alleles include: lethal - all die before end of embryonic stage; lethal - all die before end of pupal stage; some die during embryonic stage; some die during pupal stage; partially lethal; viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages.
Coordinates
chr3R:28526110-28531147:-
Coord C1 exon
chr3R:28530119-28531147
Coord A exon
chr3R:28529752-28530055
Coord C2 exon
chr3R:28526110-28529695
Length
304 bp
Sequences
Splice sites
3' ss Seq
CGTGTGGCTTTTCAATTTAGCGA
3' ss Score
7.04
5' ss Seq
GGCGTAAGT
5' ss Score
9.39
Exon sequences
Seq C1 exon
CTGAACGGCGATTGGCGCATCGACTTCCCACGCCCCATGTTCTTCGCGAACTCGTGGTGGAACTACCAACGAAAGCCAATGGGCTTCGCCGCTCCCGACCAACTGACCTGCAGCGGTCCCATCTCGGAGAGCCTCTTCATAGTTATGTTGGTGCAGGAGAAGAACATCAGCCTCGACTACGAGTACAGCATTCCGGAGAGCCTTAGTCACTCGCAGCAGGATACGCACACATGGACGCATCACCAGTTCAATGCCTGCAGTGCTTCCTGCGGCGGTGGCTCTCAGAGTCGAAAGGTCACATGCAACAATCGCATCACCTTGGCGGAAGTCAATCCATCGCTGTGCGACCAAAAGTCCAAGCCAGTGGAGGAACAGGCTTGCGGCACGGAACCGTGTGCACCACACTGGGTTGAGGGAGAGTGGTCCAAGTGCTCTAAGGGCTGCGGGTCCGATGGCTTCCAAAACAGGAGCATCACCTGCGAGCGAATCTCCTCTTCGGG
Seq A exon
CGAGCATACAGTTGAAGAAGATGCCGTTTGCCTAAAGGAGGTGGGCAACAAGCCGGCCACCAAACAGGAGTGCAACCGCGATGTAAAGAACTGCCCCAAGTATCATTTAGGACCTTGGACGCCGTGTGATAAGCTTTGCGGCGACGGAAAGCAGACACGAAAGGTGACCTGCTTCATCGAGGAGAACGGACACAAACGAGTCCTGCCCGAAGAAGATTGCGTGGAGGAGAAGCCAGAGACGGAGAAGTCCTGCCTCTTAACGCCTTGTGAGGGCGTTGACTGGATAATCTCCCAATGGAGCGGC
Seq C2 exon
TGCAATGCGTGTGGTCAGAATACCGAAACACGTACCGCCATCTGTGGCAACAAGGAAGGTAAGGTGTATCCGGAGGAATTCTGTGAACCAGAGGTACCCACTCTATCCCGGCCGTGCAAATCCCCCAAGTGCGAGGCGCAATGGTTCTCCTCGGAGTGGAGCAAGTGCTCTGCTCCTTGCGGAAAAGGTGTCAAATCTCGCATTGTCATTTGCGGGGAGTTCGATGGCAAGACGGTAACTCCAGCAGATGATGACTCTAAGTGCAACAAAGAAACGAAACCGGAATCAGAACAGGATTGTGAGGGCGAGGAGAAGGTTTGCCCCGGCGAATGGTTCACTGGACCTTGGGGCAAGTGCAGCAAGCCTTGTGGTGGGGGTGAGCGAGTCCGTGAGGTCCTGTGTCTTTCCAATGGAACCAAGTCAGTCAACTGCGATGAAGAAAAGGTCGAACCTCTATCTGAAAAGTGTAACTCTGAAGCCTGCACTGAGGATGAGATTTT
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0003137-'4-4,'4-3,5-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.003 A=0.000 C2=0.486
Domain overlap (PFAM):
C1:
PF0009014=TSP_1=PD(45.6=7.6),PF059869=ADAM_spacer1=WD(100=33.7),PF0009014=TSP_1=WD(100=16.0),PF0009014=TSP_1=PU(52.5=9.0)
A:
PF0009014=TSP_1=PD(45.8=26.5),PF0009014=TSP_1=WD(100=53.9)
C2:
PF0009014=TSP_1=WD(100=4.7),PF0009014=TSP_1=WD(100=4.2),PF0001418=Kunitz_BPTI=WD(100=4.4),PF0001418=Kunitz_BPTI=WD(100=4.4)
Main Inclusion Isoform:
FBpp0291051
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0289566, FBpp0291052, FBpp0291053
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
ACGAGTACAGCATTCCGGAGA
R:
ATTCTGACCACACGCATTGCA
Band lengths:
343-647
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)