DreEX0024945 @ danRer10
Exon Skipping
Gene
ENSDARG00000090428 | ctrb1
Description
chymotrypsinogen B1 [Source:ZFIN;Acc:ZDB-GENE-030131-1171]
Coordinates
chr7:34796558-34806805:+
Coord C1 exon
chr7:34796558-34796621
Coord A exon
chr7:34804725-34804828
Coord C2 exon
chr7:34806672-34806805
Length
104 bp
Sequences
Splice sites
3' ss Seq
GTCATTTTTTGCTCTGTCAGGCT
3' ss Score
6.58
5' ss Seq
CAGGTAAAA
5' ss Score
8.59
Exon sequences
Seq C1 exon
GGTGACTCAGAAATGGCTTTCGTCTGGATTTTGTCGTGCCTTGCCTTGATTGGAACAGCATATG
Seq A exon
GCTGTGGCGTTCCTGCCATCCCTCCAGTTGTTACCGGCTACGCCAGGATTGTGAATGGTGAGGAGGCTCGTCCTCATTCCTGGCCTTGGCAGGTGTCTCTGCAG
Seq C2 exon
CTCCCGATACTCCTGCCCTGCTCCAGCAGGCCGCTCTGCCTCTGCTGACCAATGATGACTGCAAGCGTTACTGGGGAACCAACATCACTGATCTGATGATCTGCGCTGGAGCTTCTGGTGTCTCTTCTTGCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000090428_MULTIEX1-5/8=C1-C2
Average complexity
ME(4-5[100=100])
Mappability confidence:
90%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NA
A:
PF0008921=Trypsin=PU(8.1=51.4)
C2:
PF0008921=Trypsin=FE(19.7=100)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTGACTCAGAAATGGCTTTCG
R:
TGCAAGAAGAGACACCAGAAGC
Band lengths:
196-300
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]