DreEX0060826 @ danRer10
Exon Skipping
Gene
ENSDARG00000015971 | rapgef1b
Description
Rap guanine nucleotide exchange factor (GEF) 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1001]
Coordinates
chr21:4023520-4036214:+
Coord C1 exon
chr21:4023520-4023746
Coord A exon
chr21:4026422-4026583
Coord C2 exon
chr21:4036128-4036214
Length
162 bp
Sequences
Splice sites
3' ss Seq
TTTCTCCTGTTGTGCTTCAGGCC
3' ss Score
10.62
5' ss Seq
GTGGTGAGT
5' ss Score
8.95
Exon sequences
Seq C1 exon
TCCTGCAATACATGCAGTTTGTGGAGGATTACTCTGAGCCGCAGCCGTCCGTCTTCTACCAGCTGCCTCAGAGCGAGAGTATTTACGAGCAGCGCAGGAACAAGCGCTTTCAGGAGGTTTACGGCCTCAACGACTCCTACAGCAGCTCCGACTCCATTCCTGCTTCAGCTGCAGATCCGCACGAACGGGTCCCACCACCAGCGCTGCCTCCAAAACTCAGACAACTG
Seq A exon
GCCTCCAACACTACTACTTTTCCTCCTTCATCCTCCTCTTCTGCTTTTTCAACTCTTCCTCCTCCTGCTGGGCTTCCGGAGGAGGTGGAGCCTGCTCTTGGTCTCAGCCTATCAGTGTCTAGCTCTTTCCTCAGTGGCCACGCCTCTTTGACCACTCCTGTG
Seq C2 exon
AGCGAAAGCATTGATGAAAGTGGTGAAGGAGAATATGTAAACCTGTACTCGTCCACACAGGCCAACGGAGAAAACACACACCGCACT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015971_MULTIEX2-1/2=C1-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (No Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.801 A=0.685 C2=0.951
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGCCTCAGAGCGAGAGTAT
R:
AGTGCGGTGTGTGTTTTCTCC
Band lengths:
253-415
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]