DreEX0065052 @ danRer10
Exon Skipping
Gene
ENSDARG00000011163 | sema3fa
Description
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fa [Source:ZFIN;Acc:ZDB-GENE-050513-1]
Coordinates
chr6:42912611-42914282:+
Coord C1 exon
chr6:42912611-42912652
Coord A exon
chr6:42913865-42914022
Coord C2 exon
chr6:42914215-42914282
Length
158 bp
Sequences
Splice sites
3' ss Seq
TCCTGTCGTCCTGTGTTCAGCAA
3' ss Score
7.04
5' ss Seq
AAGGTGATT
5' ss Score
5.88
Exon sequences
Seq C1 exon
GTTCCAACTTCAATCACTACTATGAAGATTTCTCCAAAACGA
Seq A exon
CAACAGCTGTATGTCAGTTCGGCGGTGGGCGTCACACATCTGGCTCTGCACAGATGTGATGTTTACGGAGAGGCCTGTGCCGACTGCTGCCTGGCCAGAGACCCATACTGTGCCTGGGACGGAAAGTCCTGCTCTCGCTACTCAGCAAACCAAAAAAG
Seq C2 exon
GCGGAGTCGAAGGCAAGACGTGAAGTATGGAAACCCTATAAGGCAATGCAGAGGCTACAACTCGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000011163-'18-21,'18-20,19-21
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.167
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(2.8=100)
A:
PF0143720=PSI=PU(62.5=66.0)
C2:
PF0143720=PSI=PD(35.7=83.3),PF0768612=V-set=PU(0.1=0.0)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCAACTTCAATCACTACTATGAAGA
R:
CATTCGAGTTGTAGCCTCTGCA
Band lengths:
108-266
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]