DreEX6066458 @ danRer10
Exon Skipping
Gene
ENSDARG00000003420 | chrna5
Description
cholinergic receptor, nicotinic, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-050417-440]
Coordinates
chr18:40503830-40518366:-
Coord C1 exon
chr18:40518258-40518366
Coord A exon
chr18:40504818-40504969
Coord C2 exon
chr18:40503830-40503874
Length
152 bp
Sequences
Splice sites
3' ss Seq
AAGTCAATGTTTTCCTCAAGGTC
3' ss Score
5.06
5' ss Seq
GTGGTGAGT
5' ss Score
8.95
Exon sequences
Seq C1 exon
CTTCTCTGTGGTTTTGCTGCTGCTGCTGAGGGTATCATGGTAACAGCTCTCAGCTTGGTCAGAGCTCTCCTCCCGCTGGTCTGTTCTGCTGTCCTGTGCTCCGCTATAG
Seq A exon
GTCCTCCTATGCTGTCCTCCTATGCCAAGACTGAAGACAAGCTGTTTAAACATCTCTTCAGCAACTACCAGAAATGGGTGAGACCAATCGAAGATCTAAACACAACTATTCAAGTAAAGTTTGGCCTGGCAATTTCCCAGCTGGTGGATGTG
Seq C2 exon
GATGAGAAGAACCAGCTGATGACGACCAATGTGTGGATGAAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000003420-'1-6,'1-5,5-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0293118=Neur_chan_LBD=PU(19.1=76.5)
C2:
PF0293118=Neur_chan_LBD=FE(6.9=100)
Main Skipping Isoform:
ENSDART00000159809fB1929
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTCTCTGTGGTTTTGCTGCTG
R:
CTGCTTCATCCACACATTGGTC
Band lengths:
154-306
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]