DreEX6069713 @ danRer10
Exon Skipping
Gene
ENSDARG00000020876 | pdk2a
Description
pyruvate dehydrogenase kinase, isozyme 2a [Source:ZFIN;Acc:ZDB-GENE-120910-1]
Coordinates
chr12:28453163-28453839:-
Coord C1 exon
chr12:28453768-28453839
Coord A exon
chr12:28453342-28453526
Coord C2 exon
chr12:28453163-28453252
Length
185 bp
Sequences
Splice sites
3' ss Seq
ACACTTCATTGTGCACACAGGTT
3' ss Score
7.06
5' ss Seq
ACAGTAAGT
5' ss Score
9.49
Exon sequences
Seq C1 exon
GTATATTCAGAGTTTGATGGAGATCTTGGAATTCTTGGAGAAAAGTCCAGATGACCACAGTATATTAGAAGA
Seq A exon
GTTTGTCGCTGCTCTGGTCAACATCCGCAACAGACACAATGATGTTGTACCCACCATGGCCCAGGGCGTGATTGAATACAAAGAGGTATTTGGTCAAGATCCAGTCACTAATCAGAATATCCAGTATTTCCTGGACCGTTTCTATTTGAGCCGGATATCCATCCGAATGCTAATCAACCAGCACA
Seq C2 exon
CTCTTGTATTTGATGGAGCCACAAATCCAGTTCACCCCAATACCATTGGCAGTATAGACCCTCACTGTCAAGTGGCAGACGTTGTTAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000020876-'5-3,'5-2,6-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF104364=BCDHK_Adom3=FE(14.5=100)
A:
PF104364=BCDHK_Adom3=FE(37.6=100)
C2:
PF104364=BCDHK_Adom3=PD(12.1=64.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
ATTCAGAGTTTGATGGAGATCTTGGA
R:
AACAACGTCTGCCACTTGACA
Band lengths:
154-339
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]