DmeEX6009465 @ dm6
Exon Skipping
Gene
FBgn0017558 | Pdk
Description
The gene Pyruvate dehydrogenase kinase is referred to in FlyBase by the symbol DmelPdk (CG8808, FBgn0017558). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (3 unique). Gene sequence location is 2R:9426439..9434638. Its molecular function is described by: protein serine/threonine kinase activity; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity. It is involved in the biological process described with: pyruvate metabolic process; negative regulation of pyruvate dehydrogenase activity; glucose homeostasis. 17 alleles are reported. No phenotypic data is available. The phenotypic classes of alleles include: fertile; viable; female semi-sterile. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 and 18-24 hour embryonic stages, during late larval stages, during late pupal stages.
Coordinates
chr2R:9431971-9433064:+
Coord C1 exon
chr2R:9431971-9432178
Coord A exon
chr2R:9432235-9432425
Coord C2 exon
chr2R:9432490-9433064
Length
191 bp
Sequences
Splice sites
3' ss Seq
TAATCACATTTGCCTTTCAGGTT
3' ss Score
8.78
5' ss Seq
ACAGTAAGA
5' ss Score
5.96
Exon sequences
Seq C1 exon
GTCAGAATGCCTGCGAGAAGAAATCGTACATATTCCTGCGCAAGGAACTGCCTGTTCGACTGGCGAATATCATGAAGGAGATCGCCCTTCTGCCGGATAACCTGTTGCACACCAGATCCGTAAGCGAAGTTAGTTCCTGGTATGTCAAGAGTTTCGAGGATGTGCTGGTGTACGAGAAAGCAGAGCCCACCCACGACAATCTGCAAAA
Seq A exon
GTTTGTGGCCGATTTGGATCTCATTAGGAATCGGCACAACGATGTGGTGCAAACGATGGCCCAGGGTGTGATCGAAATGAAGGAGAACGAGGGCGGCCAGGTGGATGCGCCTACAGAGAGTTCCATTCAGTACTTTCTCGACAGGCTCTACATGTCTCGCATCAGCATTCGAATGCTGATAAATCAGCACA
Seq C2 exon
CCCTTCTCTTTGGCGGAAATCCACATGCGGGTGGCCGCCATATTGGTTGCCTGGATCCCGCCTGTGATCTGTCGGATGTGGTTCGCGATGCTTACGAGAATGCGCGATTCCTATGCGATCAGTACTACCTGACCAGTCCGGCATTGGAGATCCAGCAGCACAGCAGCGAGCCTGGAGACAACCTGCCCATCCGCACCGTTTACGTGCCCTCGCACCTGTACTACATGCTTTTCGAGCTCTTCAAGAACTCTATGCGAGCTGTTGTAGAGCACCATGGCCATGACAACAATGACACATTGCCCCCCTTGAAAGTAGCCATTTGCAAGGGCAAGGAGGACATCTGTGTGAAAATTTCTGACCAGGGAGGCGGCATTCCCCGCTCCCAGACCGATCAGCTCTTCAAGTATATGTACAGCACAGCGCCGCAGCCCTCGAAGTCGGATCTGCATACGGTGCCATTAGCGGGCTATGGATACGGTCTGCCGATCTCAAGGCTTTAC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0017558-'8-2,'8-1,9-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.108 C2=0.042
Domain overlap (PFAM):
C1:
PF104364=BCDHK_Adom3=FE(42.1=100)
A:
PF104364=BCDHK_Adom3=FE(39.0=100)
C2:
PF104364=BCDHK_Adom3=PD(11.6=9.9),PF0251821=HATPase_c=PU(97.6=64.6)
Main Inclusion Isoform:
FBpp0111805
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0087592, FBpp0297719, FBpp0305219
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TTCTGCCGGATAACCTGTTGC
R:
ACTGATCGCATAGGAATCGCG
Band lengths:
244-435
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)