DreINT0038772 @ danRer10
Intron Retention
Gene
ENSDARG00000075883 | bub3
Description
BUB3 mitotic checkpoint protein [Source:ZFIN;Acc:ZDB-GENE-041010-210]
Coordinates
chr12:49193302-49194502:-
Coord C1 exon
chr12:49194286-49194502
Coord A exon
chr12:49193487-49194285
Coord C2 exon
chr12:49193302-49193486
Length
799 bp
Sequences
Splice sites
5' ss Seq
CAAGTGAGT
5' ss Score
9.1
3' ss Seq
GTGTGTTCTGGTGTTCACAGATC
3' ss Score
9.97
Exon sequences
Seq C1 exon
GTGGTTCAGACGGTTTCGTCAACATCTGGGACCCGTTCAATAAGAAGCGTCTGTGTCAGTTCCACCGGTACCCCAGCAGCATCGCCTCTCTGTCCTTCAGCACGGACGGCTCTCTGCTGGCCATCGCCTCCTCATACATGCAGGAGCTGGGGGACGTCTCACACCCCGCTGACGCTGTGTTCATCCGCCAGGTCACCGATGCAGAGACCAAGCCCAA
Seq A exon
GTGAGTCACACACACACACACACACACACACACACACACACACACAAGCTGAGGCTGAGTGTGTGTGTTCAGTGATGCTCCTGCGTGCGTGCGTGCGTGCGTGTGTGTGTGCGCGTGTGTGTGTTTGGGTTAGTGAACTGTTGTTTGGCTTTAGATTATCGACTGTCCTCACTGCTGGATTATTGCAGTAAATCTCTCCTCATTCATTATCGCTGAACATCTGACCGTGTTGTGTGCTTGTCGAGACCAGGGCTTCCCAAAGTCGGGGTTGCAGGACAATGACGGGGGGTCACTTGGTGATTTCTGAAAGTCGAATAAATCACTCCGTGACACCTTTATCCATCAAACACATTAAACATGAACACTGAGGACCGTCTCTGAGTCTCCAAAACTAAAGCAACATCCGTCTTGTGCTGAGTCCAGCGCTGTCATTAGCGGACGCTAGCATTAATACTGCAGTGATCTAGCGATGATGAGGGTTTAGACTGTTGTGTGCTGTCAGTGTGTGTGTGTGTGTGTGTGTGCTGTCAGTGTGTGTGTGTGTGTGTTCAGTGTTTGTATATTTATAATTGGCTCTGAGGAATGTGGGGGTCTCGAGTCGCTGGCCTTGTTGTTTTTGGGGGTCACGAGCTGAAAAGTTTGGGAACCCCTGATCTAGAAGATGCTGCGTGATTTAAGAGTTTACATATCTGGACTAGAAACAAGACACACACTCTCAGTGACAAGCAGTGTGTGTGTGTGTGTGTTTATATACTCATGTGATGTGTGTGTGTGTGTGTGTGTGTTCTGGTGTTCACAG
Seq C2 exon
ATCGACGTGATTGGCTGAGCTGCAGTGATTACCCCTGAACACACCCCTCCTCCACCACTCTGCCTGCCTGTCTGTCTAACGTGTGTGTATATCTGTGTGTGTGTGTGTGTGTGTGTGTTCTGTTCTCCAGTGTGTTTTTATGTTCTGAAAATCAATAAATCTTTTTTTTATTCATGTAACGTGCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075883:ENSDART00000153244:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.027 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0040027=WD40=PD(32.3=13.7)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCAACATCTGGGACCCGTTC
R:
TCAGAACATAAAAACACACTGGAGA
Band lengths:
348-1147
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]