Special

MmuEX0027468 @ mm9

Exon Skipping

Gene
ENSMUSG00000028649 | Macf1
Description
microtubule-actin crosslinking factor 1 [Source:MGI Symbol;Acc:MGI:108559]
Coordinates
chr4:123177116-123179532:-
Coord C1 exon
chr4:123179407-123179532
Coord A exon
chr4:123177996-123178001
Coord C2 exon
chr4:123177116-123177268
Length
6 bp
Sequences
Splice sites
3' ss Seq
AATCATGCTCCCGGGGACAGGCA
3' ss Score
3.97
5' ss Seq
CAGGTAGTT
5' ss Score
6.3
Exon sequences
Seq C1 exon
TTCTTCAGTGATGCCCGAGACCTGGAGTCATTTCTGAGAAACCTCCAAGACTCCATCAAACGAAAATATACCTGCGACCGCAGCACCAGTCTGTCCCGCCTGGAGGATCTGCTTCAGGACTCCATG
Seq A exon
GCACAG
Seq C2 exon
GATGAAAAGGAGCAGCTTATTCAGTCCAAGAGTTCCGTTGCCAGTCTTGTTGGGAGATCAAAAACCATTGTTCAGCTAAAGCCACGCAATCCAGACCATGTGCTGAAGAGCACTTTATCTGTGAAGGCCATCTGTGACTACCGGCAGATTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000028649-MICROEX3
Average complexity
MIC_S
Mappability confidence:
NA
Protein Impact





	

	
Alternative protein isoforms (No Ref)





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.002
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
NO

							
A:
NO

							
C2:
NO

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000081324f6186A





     
                   









    
Main Skipping Isoform:
ENSMUSP00000081324f2416A





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000080755, ENSMUSP00000081324, ENSMUSP00000095508, ENSMUSP00000095509, ENSMUSP00000101827, ENSMUSP00000101831, ENSMUSP00000101831f2417A, ENSMUSP00000114568, ENSMUSP00000115847, ENSMUSP00000123246
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr1:39299252-39299257 
LOW PSI
HsaEX0037159
(MACF1)
Human
(hg19)
chr1:39764924-39764929 
LOW PSI
HsaEX0037159
(MACF1)
Rat
(rn6)
chr5:141182257-141182262 
ALTERNATIVE
RnoEX0051761
(Macf1)
Cow
(bosTau6)
chr3:107325584-107325589 
ALTERNATIVE
BtaEX0021014
(MACF1)
Chicken
(galGal4)
chr23:5331525-5331530 
ALTERNATIVE
GgaEX0029074
([1])
Chicken
(galGal3)
chr23:5646168-5646173 
ALTERNATIVE
GgaEX0029074
(MACF1)
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CGAAAATATACCTGCGACCGC

				
R: 
GCAACGGAACTCTTGGACTGA

				
Band lengths: 
107-113

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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