Special

MmuEX0033853 @ mm9

Exon Skipping

Gene
ENSMUSG00000031860 | Pbx4
Description
pre B cell leukemia homeobox 4 [Source:MGI Symbol;Acc:MGI:1931321]
Coordinates
chr8:72356570-72390502:+
Coord C1 exon
chr8:72356570-72356750
Coord A exon
chr8:72382979-72383052
Coord C2 exon
chr8:72390367-72390502
Length
74 bp
Sequences
Splice sites
3' ss Seq
GCCTTTGCTGCCTCTTTCAGAAA
3' ss Score
11.01
5' ss Seq
CTGGTAGGC
5' ss Score
6.99
Exon sequences
Seq C1 exon
GGGACCTGTCCCTCTGCGCTCGCTCGGGACTGTAGCCCATGGCCGCCCCTCTGCGTCCCGTGCCGCCACAGCCCGCCCCGCGCCGCCTGCCGACCACAGCTCCACTAGGCCATGACACGAGCGACGTCCTGCAGCAGATCATGGCCATCACCGACCAGAGCCTGGACGAGGCTCAGGCCAG
Seq A exon
AAAGCATGCCCTGAACTGCCATCGGATGAAGTCTGCTCTCTTCAGTGTGCTCTGCGAGATCAAGGGGAAGACTG
Seq C2 exon
GCGCAAGCGGCGGAATTTCAGCAAGCAGGCCACAGACGTGCTGAATGAATATTTCTATTCCCATTTGAGCAACCCTTACCCCAGTGAAGAGACCAAAGAAGAGCTGGCCAGGAAGGGTGGCATCACAGTGTCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031860_MULTIEX1-1/5=C1-4
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.881 A=0.116 C2=0.302
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF037928=PBC=PU(14.8=60.4),PF070286=DUF1319=PU(7.5=27.1)

							
A:
PF037928=PBC=FE(12.8=100),PF070286=DUF1319=FE(14.4=100)

							
C2:
PF040917=Sec15=PD(0.1=0.0),PF083976=IMD=PD(26.8=71.7),PF0004624=Homeobox=PU(75.0=97.8)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000122281





     
                   









    
Main Skipping Isoform:
NA





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000080219, ENSMUSP00000118287, ENSMUSP00000119526, ENSMUSP00000121369
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr19:19599292-19599365 
ALTERNATIVE
HsaEX0045761
(PBX4)
Human
(hg19)
chr19:19710101-19710174 
ALTERNATIVE
HsaEX0045761
(PBX4)
Rat
(rn6)
chr16:21316099-21316172 
HIGH PSI
RnoEX6055345
(Pbx4)
Cow
(bosTau6)
chr7:3652831-3652904 
HIGH PSI
BtaEX0026044
(PBX4)
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
chrUn_random:30310589-30310662 
HIGH PSI
GgaEX6047764
(PBX4)
Zebrafish
(danRer10)
chr3:52756828-52756901 
HIGH PSI
DreEX0053815
(pbx4)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
CAGATCATGGCCATCACCGAC

				
R: 
GACACTGTGATGCCACCCTTC

				
Band lengths: 
179-253

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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