MmuEX0033857 @ mm9
Exon Skipping
Gene
ENSMUSG00000031860 | Pbx4
Description
pre B cell leukemia homeobox 4 [Source:MGI Symbol;Acc:MGI:1931321]
Coordinates
chr8:72390367-72394583:+
Coord C1 exon
chr8:72390367-72390502
Coord A exon
chr8:72393928-72394081
Coord C2 exon
chr8:72394477-72394583
Length
154 bp
Sequences
Splice sites
3' ss Seq
GTCGGTCCATCTATCCACAGGTC
3' ss Score
10.48
5' ss Seq
CAGGTGACG
5' ss Score
7.66
Exon sequences
Seq C1 exon
GCGCAAGCGGCGGAATTTCAGCAAGCAGGCCACAGACGTGCTGAATGAATATTTCTATTCCCATTTGAGCAACCCTTACCCCAGTGAAGAGACCAAAGAAGAGCTGGCCAGGAAGGGTGGCATCACAGTGTCCCAG
Seq A exon
GTCTCTAACTGGTTTGGCAACAAAAGAATCCGGTACAAAAAGAATACAGGGAAGTTTCAGGAAGAAGCCACCATGTACACAGGGAAAGCATCCACAGTCACCAAGGCCCGGCGTCCCAGGGGTCAGAGCAGCTGCCAGTCCACACCCAGCCCAG
Seq C2 exon
GTCCCTGTGGCCCCTTGCCACTGACCAATGGAAGTGACGTGGTTCTCACTCTGCGGACCCTGGCCTTCCTCCAGCCCCCCACTGGGGGAGTCTGCCTACAGCCCCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000031860_MULTIEX1-5/5=4-C2
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
Show structural model
Features
Disorder rate (Iupred):
C1=0.302 A=0.510 C2=0.194
Domain overlap (PFAM):
C1:
PF040917=Sec15=PD(0.1=0.0),PF083976=IMD=PD(26.8=71.7),PF0004624=Homeobox=PU(75.0=97.8)
A:
PF0004624=Homeobox=PD(21.7=25.0)
C2:
PF037928=PBC=PD(3.5=5.4)
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAAGCGGCGGAATTTCAGCAA
R:
CTGTAGGCAGACTCCCCCAG
Band lengths:
234-388
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types