RnoEX0053022 @ rn6
Exon Skipping
Gene
ENSRNOG00000010737 | Mbnl2
Description
muscleblind-like splicing regulator 2 [Source:RGD Symbol;Acc:1591755]
Coordinates
chr15:105756930-105764288:+
Coord C1 exon
chr15:105756930-105757049
Coord A exon
chr15:105763422-105763475
Coord C2 exon
chr15:105764135-105764288
Length
54 bp
Sequences
Splice sites
3' ss Seq
TCGCTGCCTGTTATTAAAAGACT
3' ss Score
2.9
5' ss Seq
CTGGTACTT
5' ss Score
4.74
Exon sequences
Seq C1 exon
GAGAAATGCAAATATTTTCACCCTCCTGCACACTTGCAGGCCAAAATCAAAGCTGCGCAGCACCAAGCCAACCAGGCCGCGGTGGCCGCCCAGGCAGCCGCGGCCGCGGCCACAGTCATG
Seq A exon
ACTCAGTCGACTGCCAAAGCACTGAAGCGACCTCTCGAAGCAACTGTAGACCTG
Seq C2 exon
GCCTTCCCTCCCGGTGCTCTTCACCCTTTACCAAAGAGACAAGCACTTGAAAAAAGCAACGGAGCCAGCACGGTCTTCAACCCCAGCGTCTTGCACTACCAGCAGGCTTTGACCAGTGCGCAGTTGCAGCAACACACGGCGTTCATTCCCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000010737_MULTIEX1-5/5=4-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (disopred):
C1=NA A=NA C2=NA
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
TTTCACCCTCCTGCACACTTG
R:
TTGGTAAAGGGTGAAGAGCAC
Band lengths:
139-193
Functional annotations
There are 2 annotated functions for this event
PMID: 21454535
Inferred from MBNL1: The exon 5 and 6 regions are both needed to control the nuclear localization of MBNL1
PMID: 27733504
The presence of alternative ex.54nt always significantly elevated MBNL1 and MBNL2 protein levels (_2.7 and _4.0-times, respectively). There were marginal changes of the GFP-MBNL level for constructs carrying ex.36nt (_1.3-times) and ex.95nt (_0.8-times). There were no significant differences between the activities of the analyzed proteins for the majority (67%) of AS events. However, these exons might have either a positive or negative effect on some specific AS events (Figure 5A and C). Interestingly, exOFF events predominated in AS events with a negative effect of ex.36nt (95%; P = 0.002) and a positive effect of ex.95nt (90%; P = 0.010). All together, these data indicate that the presence of sequences encoded by these alternative exons can significantly modulate MBNL splicing activity, but this regulation depends strongly on the targeted RNA.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]