Special

DmeEX0004917 @ dm6

Exon Skipping

Gene
FBgn0063485 | Lasp
Description
The gene Lasp is referred to in FlyBase by the symbol DmelLasp (CG3849, FBgn0063485). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (4 unique). Gene sequence location is 3L:16676406..16711677. Its molecular function is described by: protein binding; actin filament binding; actin binding. It is involved in the biological process described with 7 unique terms, many of which group under: regulation of multicellular organismal process; cellular component assembly involved in morphogenesis; regulation of system process; regulation of myofibril size; positive regulation of oocyte development. 41 alleles are reported. The phenotypes of these alleles manifest in: germline cyst; adult integumentary system; external sensory organ; spermatid cyst; adult. The phenotypic classes of alleles include: phenotype; lethal - all die before end of P-stage; increased mortality during development; lethal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages.
Coordinates
chr3L:16681238-16711677:-
Coord C1 exon
chr3L:16710943-16711677
Coord A exon
chr3L:16694202-16694296
Coord C2 exon
chr3L:16681238-16681370
Length
95 bp
Sequences
Splice sites
3' ss Seq
CCTCTTTTCTTTCGTTTCAGACA
3' ss Score
11.14
5' ss Seq
GGCGTAAGT
5' ss Score
9.39
Exon sequences
Seq C1 exon
CAGCGCAGTCGCGACAGCTGAAAATTAATAAAACGAAGAGAAAAGTGTAAATTGGATCACAATTGGTAACAAAAAGTCTATGCGATCGATCGCCAGTTGTTTTATACATCAACAACAACAAAGCAAACCAGTTATTAGATGTTTTTGTGAATAGTGTAAATCAAATTCTCGGCTCATACCTACACACACACCAAATACATTCCCGTAGTTCCTATTCAGATCCGATCGGTTCTTCGACTCGCAGATCACAGAAAATATGCAAAATTAAGCCAAGAGGAGCGTAAATTCGTGCGCAAATCGTGGGCGCAAAAGTGTCGTCAAAACGGGGAAAATAATCAAAGTTAACAGTAATATCCTGTTGTGTAAATAAAAGGCTTATACATACACACACAGTCGCAGAAGCAACAAACAAAGGAGGAAAAAGCGGCAAGATGAATAAAACCTGTGCCCGTTGCCAAAAGGTGGTTTACCCCATCGAGGAGCTTAAGTGTCTGGACAAA
Seq A exon
ACATGGCACAAAACGTGTTTCAAATGCACAGAATGCGGCATGACGCTCAATATGAAAACCTACAAGGGCTACAACAAAATGCCCTACTGCGAGGC
Seq C2 exon
ACACATCCCGAAGGCGAAGGCAACGGCCATTGCCGATACGCCCGAATTGAAGCGCATCGCGGAGAACACCAAAATCCAGTCCAACGTCAAGTACCACGCGGACTTTGAGAAGGCCAAGGGCAAATTTACACAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0063485_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact

ORF disruption upon sequence exclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.283
Domain overlap (PFAM):

C1:
PF0041217=LIM=PU(31.6=78.3)
A:
PF0041217=LIM=FE(54.4=100)
C2:
PF0041217=LIM=PD(10.5=13.3),PF0088013=Nebulin=WD(100=64.4)


Main Inclusion Isoform:
FBpp0099495


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0075109, FBpp0297281, FBpp0297282


Other Skipping Isoforms:
NA
Associated events
Primers PCR
Suggestions for RT-PCR validation
F:
ACAAAGGAGGAAAAAGCGGCA
R:
GGATTTTGGTGTTCTCCGCGA
Band lengths:
169-264
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)