Special

DmeEX0009046 @ dm6

Exon Skipping

Gene
FBgn0031681 | pgant5
Description
The gene Polypeptide N-Acetylgalactosaminyltransferase 5 is referred to in FlyBase by the symbol DmelPgant5 (CG31651, FBgn0031681). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (2 unique). Gene sequence location is 2L:5072170..5093592. Its molecular function is described by: polypeptide N-acetylgalactosaminyltransferase activity. It is involved in the biological process described with: oligosaccharide biosynthetic process. 17 alleles are reported. The phenotypes of these alleles manifest in: embryonic/larval copper cell; embryonic/larval gut. The phenotypic classes of alleles include: viable; lethal; partially lethal - majority die. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed during late pupal stages.
Coordinates
chr2L:5074390-5077712:-
Coord C1 exon
chr2L:5077535-5077712
Coord A exon
chr2L:5077077-5077257
Coord C2 exon
chr2L:5074390-5074529
Length
181 bp
Sequences
Splice sites
3' ss Seq
CGACGATGATAAACCCGCAGATT
3' ss Score
4.78
5' ss Seq
CAGGTTCGC
5' ss Score
5.28
Exon sequences
Seq C1 exon
GTACCGAGTGCCATCGCGGGAAATGGCGCGCCGGAATAACGATAGGACTGCTCCGTTGCGAACTCCGACCATGGCCGGTGGTCTGTTCTCCATCGATAAGGATTACTTCTATGAGATTGGCTCCTACGACGAGGGCATGGACATTTGGGGCGGCGAAAATCTGGAGATGTCGTTCCGT
Seq A exon
ATTTGGCAGTGCGGCGGCATTTTAGAAATAATACCCTGCTCCCACGTTGGCCACGTGTTCCGTGACAAATCGCCGTACACCTTCCCCGGCGGCGTGGCCAAAATTGTGCTGCACAATGCGGCCCGGGTGGCCGAAGTTTGGCTGGACGAGTGGCGTGATTTCTATTATTCGATGAGCACAG
Seq C2 exon
GAGCTCGAAAGGCATCGGCTGGCGATGTGAGCGATCGTAAAGCTCTGCGAGATCGCCTCAAATGCAAGAGTTTCCGCTGGTATCTGGAGAACGTTTATCCCGAGAGTTTAATGCCCTTGGATTACTACTATCTGGGCGAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0031681_MULTIEX1-1/2=C1-C2
Average complexity
ME(1-2[99=100])
Mappability confidence:
100%=100=100%
Protein Impact

ORF disruption upon sequence exclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.100 A=0.000 C2=0.000
Domain overlap (PFAM):

C1:
PF0053521=Glycos_transf_2=PD(19.5=60.0)
A:
PF071836=DUF1403=PU(65.2=70.5)
C2:
PF071836=DUF1403=PD(33.3=46.8),PF0065217=Ricin_B_lectin=PU(1.7=4.3)


Main Inclusion Isoform:
FBpp0078663


Main Skipping Isoform:
FBpp0110323


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
NA
Primers PCR
Suggestions for RT-PCR validation
F:
GCGCCGGAATAACGATAGGAC
R:
CGTTCTCCAGATACCAGCGGA
Band lengths:
244-425
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)