DreEX0059414 @ danRer10
Exon Skipping
Gene
ENSDARG00000103699 | ptpn13
Description
protein tyrosine phosphatase, non-receptor type 13 [Source:ZFIN;Acc:ZDB-GENE-070410-72]
Coordinates
chr21:9388294-9409633:-
Coord C1 exon
chr21:9409540-9409633
Coord A exon
chr21:9402695-9403258
Coord C2 exon
chr21:9388294-9388383
Length
564 bp
Sequences
Splice sites
3' ss Seq
TATTTTTCTTCCATCTTTAGGCC
3' ss Score
11.03
5' ss Seq
ATGGTGAGT
5' ss Score
10.13
Exon sequences
Seq C1 exon
CTGGATGGGCTGCTTTCCCAGACAGAAAGGGAGTCAATTCCAGAAAGAAGCAAAGAAATCCGCGAGAGACTTCGAGGAAAAGGGCTTCCTTCAG
Seq A exon
GCCGTAGTGCTGCACATCGTGTGCTGGAGCGTTACAGAGCCCGAACACAAGAGCAGGCCAACCTGAACCGTGGACTCAGCCGCTCCATGGGCTCTCTCCCTATGCAGGACCTAATCCACGCCAACGAAGGTCTCACTCCATACCCGCCTCCAGATTATCCCACAGCATCTGAGTTACCTGTCGAACTCTACCACCAACGTCAGCAACAACAACTGCAACAACAGCGTGGTCGACCCATAGAAGTGAACCAGCGCCTGCATCCACACCAGAGGAACAAGAGCTGGGCTTCTTCTGCAGATCTGGCCTGTCTGGACCCAGATATGCTTCGCTTCGGGGCTCTTGAAGACGCCCGCAGGGGTAGCAGTGCCCTGAGCACCCGTTCAGCTGGCAGACACAAGCCCTCTTCTAAAGCCAGAGACTCCCGTTACTCCGAATTTGGAAGTCTGGATGTCAGAAAGACGCACCATCATTCCAATCAATCCGTCGGCTCGGCCTTCAATAGTGCTTACGATCGCATCAGAGAGAAACAGAAGAAACTGCAAGCGCTCAAACAGGCCATTGATG
Seq C2 exon
ATCCTCATACTCACAGAAGATACAACAGTGACTACAGCTCGTCCAGTGAAAGCCCTTCGGTGACATCATCAGACCCCGACTACAGACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000103699-'8-7,'8-6,9-7
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.812 A=0.910 C2=1.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGATGGGCTGCTTTCCC
R:
TTGTCTGTAGTCGGGGTCTGA
Band lengths:
183-747
Functional annotations
There are 1 annotated functions for this event
PMID: 12529439
Here the authors show that PTP-BL is involved in the regulation of cytokinesis. The authors demonstrate localization of endogenous PTP-BL at the centrosomes during inter- and metaphase and at the spindle midzone during anaphase. Finally PTP-BL is concentrated at the midbody in cytokinesis. The authors show that PTP-BL is targeted to the midbody and centrosome by a specific splicing variant of the N-terminus characterized by an insertion of 182 amino acids (HsaEX0050973). Additionally, the splice variant with the insert leads to multinucleated cells when overexpressed in HeLas.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]