RnoEX0071169 @ rn6
Exon Skipping
Gene
ENSRNOG00000002061 | Ptpn13
Description
protein tyrosine phosphatase, non-receptor type 13 [Source:RGD Symbol;Acc:1563360]
Coordinates
chr14:7768810-7796296:-
Coord C1 exon
chr14:7796209-7796296
Coord A exon
chr14:7782502-7783047
Coord C2 exon
chr14:7768810-7768905
Length
546 bp
Sequences
Splice sites
3' ss Seq
ATAGATTCTTATCATTCCAGGAA
3' ss Score
5.73
5' ss Seq
AAGGTTAGT
5' ss Score
8.54
Exon sequences
Seq C1 exon
ACGGATCCGCTTTCCCATAGCAGTGAAGAAAAGCCTGACCGGAGCCAGGCCATCCGAGACCGACTGAGAGGAAAAGGATTACCCATAG
Seq A exon
GAAGAAGCTCCACTTCTGACGCACTAGACACACACAAGGCTCCACTCTCTCAGCAGACCTTTCTTAACAAAGGGCTTAGTAAATCTATGGGATTTCTGTCCGTCAGAGACACACAAGAGGAGGAAGATTATTCCAAGGAAATTCCCTCCGATAATAATTCCAGGCATGAAGACTCTGAGCCTTTCTCCTCCCCTTACCAGTTCAAAACTAGTAGTCCACACATTGACGTACTCTCTAAGAAGAAGACCTGGGCTTCGTCCATGGACTTGCTCTGTGCGGCCGACAGAGACCTTTCTGGAGAGATTGGAAGGTACCAACACTGTGACCCTGAGACAGGGACGGGACGGACGTCGATCACTTCTAGGAAGAAAGAAGGACGGTACTCTGACGGAAGCATTGCCTTGGATGTCTTCGGCCCTCAGAAAGCAGAGCCAGTGATCCACAGCCGAGAACTGCCGGCTTCCTCCGCTGTTTCGAGCGCTTTGGACCGAATTCGAGAGAGACAAAAGAAACTTCAGGTTCTGAGAGAAGCCATGAATGTAGAAG
Seq C2 exon
AGCCAGTCCGAAGATACAAAACTTACCACAGCGATCTGTTCAGCACTTCCAGTGAAAGCCCGTCTATTATTTCCTCGGACTCAGACTTCAGGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000002061_CASSETTE3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.933 A=0.607 C2=0.515
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGATCCGCTTTCCCATAGCAG
R:
CTTGCCTGAAGTCTGAGTCCG
Band lengths:
182-728
Functional annotations
There are 1 annotated functions for this event
PMID: 12529439
Here the authors show that PTP-BL is involved in the regulation of cytokinesis. The authors demonstrate localization of endogenous PTP-BL at the centrosomes during inter- and metaphase and at the spindle midzone during anaphase. Finally PTP-BL is concentrated at the midbody in cytokinesis. The authors show that PTP-BL is targeted to the midbody and centrosome by a specific splicing variant of the N-terminus characterized by an insertion of 182 amino acids (HsaEX0050973). Additionally, the splice variant with the insert leads to multinucleated cells when overexpressed in HeLas.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]