DreEX6056043 @ danRer10
Exon Skipping
Gene
ENSDARG00000041864 | capn3a
Description
calpain 3a, (p94) [Source:ZFIN;Acc:ZDB-GENE-040912-97]
Coordinates
chr17:45339480-45342363:+
Coord C1 exon
chr17:45339480-45339557
Coord A exon
chr17:45339649-45339809
Coord C2 exon
chr17:45342194-45342363
Length
161 bp
Sequences
Splice sites
3' ss Seq
CTCTTGTCGTCAACCTGTAGGAT
3' ss Score
8.56
5' ss Seq
CAGGTGGGT
5' ss Score
8.56
Exon sequences
Seq C1 exon
TTCTAAAGAATGGGAAAGTCTTTCTAAAGCGGAAAAAGAGAAACTCCAGCAACAGAACGCTGAAGATGGAGAGTTTTG
Seq A exon
GATGTCGTTTGAAGATTTTAAGAAAAATTACACCAAAATTGAGATTTGTAACTTGACACCTGATGCCCTGGAGGACGATAAGCTACATAAGTGGACAGTGTCTGTCAATGAGGGCCGCTGGGTGAGAGGCTGCTCTGCCGGAGGATGCAGAAACTATCCAG
Seq C2 exon
ACACATTCTGGACAAACCCACAATACCGTCTGCGTCTCCTGGAGGAAGATGACGATCCGGAGGATGATGAAGTGGCCTGCACATTTGTTGTTGCTCTAATGCAGAAAAACAGACGTAAAGAACGCAAGTTGGGTGCGAATCTCTTGACCATTGGATTTTCCATCTATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000041864-'10-9,'10-8,11-9=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.370 A=0.000 C2=0.052
Domain overlap (PFAM):
C1:
PF0064816=Peptidase_C2=FE(15.9=100)
A:
PF0064816=Peptidase_C2=PD(11.6=34.5),PF0106717=Calpain_III=PU(15.5=43.6)
C2:
PF0106717=Calpain_III=FE(36.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGAATGGGAAAGTCTTTCTAAAGCG
R:
GGAAAATCCAATGGTCAAGAGATTCG
Band lengths:
234-395
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]