DreEX6083491 @ danRer10
Exon Skipping
Gene
ENSDARG00000019231 | spna2
Description
spectrin alpha 2 [Source:ZFIN;Acc:ZDB-GENE-051113-60]
Coordinates
chr21:13076718-13079132:+
Coord C1 exon
chr21:13076718-13076840
Coord A exon
chr21:13076974-13077191
Coord C2 exon
chr21:13079040-13079132
Length
218 bp
Sequences
Splice sites
3' ss Seq
AATTTTCCCCCAAATTTCAGGTC
3' ss Score
8.95
5' ss Seq
GAGGTAAAC
5' ss Score
7.2
Exon sequences
Seq C1 exon
GAAAAGACCTTATTGGGGTCCAAAACCTTCTGAAGAAACACCAGGCTCTGCAGGCAGAAATCTCTGGCCACGAACCCCGAATCAAGGCTGTTACTCAGAAGGGGGAGGCCATGATTGATGAAG
Seq A exon
GTCACTTTGCAGGGGAGGATGTGAAGGCTAAACTACAAGAGCTGCACAACCGCTGGGACGGGCTGAAAGGAAAAGCAGCCCAGCGTAGACAGGATCTGGAGGACTCTCTCCAGGCTCAGCAGTACTTTGCTGATGCCAACGAGGCCGAGTCCTGGATGAGGGAGAAGGAGCCCATTGTAGGAAGCCCTGACTACGGCAAAGATGAGGACTCTGCTGAG
Seq C2 exon
GCTTTGCTGAAGAAGCATGAGGCTCTGATGTCTGATTTGAGTGCATATGGCAGCAGCATCCAGGCCTTGAAAGAAGAAGCTCAGGCCTGCAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000019231-'22-27,'22-25,23-27=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.571 A=0.603 C2=0.226
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=FE(39.0=100)
A:
PF0043516=Spectrin=PD(32.4=46.6),PF0043516=Spectrin=PU(42.9=49.3)
C2:
PF0043516=Spectrin=FE(35.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCAAAACCTTCTGAAGAAACACC
R:
GCAGGCCTGAGCTTCTTCTTT
Band lengths:
196-414
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]