DreEX0060811 @ danRer10
Exon Skipping
Gene
ENSDARG00000015971 | rapgef1b
Description
Rap guanine nucleotide exchange factor (GEF) 1b [Source:ZFIN;Acc:ZDB-GENE-040426-1001]
Coordinates
chr21:4005994-4018424:+
Coord C1 exon
chr21:4005994-4006143
Coord A exon
chr21:4016327-4016447
Coord C2 exon
chr21:4018238-4018424
Length
121 bp
Sequences
Splice sites
3' ss Seq
ATCTGCATATTCTGCCGTAGTCC
3' ss Score
6.47
5' ss Seq
CAGGTACAA
5' ss Score
7.09
Exon sequences
Seq C1 exon
TGGGTGTGAAAATCCTCTAACCGAAAGAGAGAAGGCCATCCTGAGTAAGACCACGCCCATTGTCACGCCCACTGACAGCTTGACTGACATGTCAGAGGAGGAGGCTCCACCCAAACCTCCTCTACCAGGACCCAAACTAGCAGAGCACAG
Seq A exon
TCCACCGGCTCTGCCTCCGAAGAAGCGTCAGTCGGCCCCGTCACCCACCCGTGTGGCAGTGGTGGCCCCGATGAGCCGGGGTCCGTGTAGCCTCAGCCTGCCTCCTGGAGCCCTCAGACAG
Seq C2 exon
CAGCAGGACTTTGATGTGGATCTCCTCCAGAGGCGTTTCTCAGGCGGCAGTCAGTCATATGATGGAGAATCTCCTCGTCTTTCTCCCTGTAACAGCATGGGAAAACTCAGCAAATCTGATGAGCAGCTTTCGTCTCTGGAGCGGGACAGTGGACAGTGCTCTCGCAACACCAGCTGTGAAACGCTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015971-'10-12,'10-11,12-12
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=1.000 C2=0.952
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAAGACCACGCCCATTGTCAC
R:
CTCCAGAGACGAAAGCTGCTC
Band lengths:
246-367
Functional annotations
There are 1 annotated functions for this event
PMID: 7735837
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: x-ray crystallography. ELM ID: ELMI001098; ELM sequence: PALPPK; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]