Special

DmeEX0008591 @ dm6

Exon Skipping

Gene
Description
The gene muscleblind is referred to in FlyBase by the symbol Dmelmbl (CG33197, FBgn0265487). It is a protein_coding_gene from Dmel. It has 19 annotated transcripts and 19 polypeptides (18 unique). Gene sequence location is 2R:17216549..17379376. Its molecular function is described by: RNA binding; metal ion binding; nucleic acid binding. It is involved in the biological process described with 9 unique terms, many of which group under: regulation of nucleobase-containing compound metabolic process; reproductive process; programmed cell death; apoptotic process; organic cyclic compound metabolic process. 93 alleles are reported. The phenotypes of these alleles manifest in: fascicle; larva; rhabdomere; visceral muscle; cytoskeleton. The phenotypic classes of alleles include: increased mortality during development; some die during embryonic stage; phenotype; courtship behavior defective.
Coordinates
chr2R:17352129-17365386:+
Coord C1 exon
chr2R:17352129-17352305
Coord A exon
chr2R:17359306-17359557
Coord C2 exon
chr2R:17361980-17365386
Length
252 bp
Sequences
Splice sites
3' ss Seq
ACACACGCACTCACACACAGACC
3' ss Score
4.37
5' ss Seq
CTGGTATTT
5' ss Score
3.5
Exon sequences
Seq C1 exon
GCAACAAACCCCTATCTGACCGGCATTCCGGCCAACTCGTACAGTCCGTACTACACAACGGGACACCTGGTGCCCGCCTTGCTGGGTCCGGATCCCGTGACCTCCCAACTGGGACCCGTGGTGCCCCAAACAGTGCAGGTGGCACAGCAGAAAATACCACGTTCCGACAGATTAGAG
Seq A exon
ACCTCACCTCTTGCGGCACACCATCACCAGCAACAACAACAATTGCAACACCAGCTGAACAACATCAACAACAACAACAACCACAGCACCGCTGGCGCAGCAGCCACCTCGACAACAGCAACAACAACCACAAACAACGCCGCTGCCGCCGCCGCCGCTGCTGCCGCCGCCGCTGCCGCCGCTGTCATGGGCCATCACACACTGGAAGTGGGCAAGAAGCGGGCGGCGGACACGACCGACATGTTTCCACTG
Seq C2 exon
CAATTCTCTGGCATGGTACCGTTCAAACGTCCAGCTGCCGAAAAGTCTGGCATTCCAGTTTATCAGCCCGGTGCGACCGCCTATCAGCAGCTAATGCAGCCCTACGTGCCAGTCTCATGTGAGTATCCCCAACAACAACAACAACCACCACCACAACAACAACAACAACAACAACATCAACAACAAAATGTACTACTACAACAACAACTACAATTAAGTAGCGTCATAACAACAACAAGTACAACAGCAACAGCCAGTAACAATATGATTAATAATAATATTAATAATAAAGGTATCATAAACGCTGTTATGGCTTCTACCAATATAACATCATCAGCAACAACAACAGCATCATCATCATTACTATCAGCGTTGGGCACACCACCAACGACAACAACAACAGCCATTACTAATCCTAACGATAGTGATAACGATTCCGATCGCAATCAAGACCATGACAAAAATCACGATAGTACTGCTGATGGTGAAGCTGATAATGC
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0265487_MULTIEX2-2/6=C1-4
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (No Ref)

No structure available
Features
Disorder rate (disopred):
  C1=0.435 A=0.608 C2=0.564
Domain overlap (PFAM):

C1:
PF0064219=zf-CCCH=PU(11.1=5.1)
A:
PF098064=CDK2AP=PD(71.6=86.9)
C2:
NO


Main Inclusion Isoform:
FBpp0297561


Main Skipping Isoform:
FBpp0297558


Other Inclusion Isoforms:
FBpp0086144, FBpp0297556, FBpp0302029


Other Skipping Isoforms:
FBpp0086146, FBpp0297559, FBpp0301759, FBpp0301760, FBpp0307871, FBpp0307872, FBpp0307873
Associated events
Conservation
Chicken
(galGal4)
HIGH PSI
Chicken
(galGal4)
ALTERNATIVE
Chicken
(galGal3)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Zebrafish
(danRer10)
HIGH PSI
Primers PCR
Suggestions for RT-PCR validation
F:
GCCAACTCGTACAGTCCGTAC
R:
TGTGGTGGTGGTTGTTGTTGT
Band lengths:
302-554
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)