Special

DmeEX0005333 @ dm6

Exon Skipping

Gene
FBgn0015773 | NetA
Description
The gene Netrin-A is referred to in FlyBase by the symbol DmelNetA (CG18657, FBgn0015773). It is a protein_coding_gene from Dmel. It has 4 annotated transcripts and 4 polypeptides (2 unique). Gene sequence location is X:14604012..14653928. Its molecular function is unknown. It is involved in the biological process described with 9 unique terms, many of which group under: animal organ development; cell motility; sensory organ development; tissue development; regulation of cell development. 27 alleles are reported. The phenotypes of these alleles manifest in: axon; photoreceptor cell R8 stalk; cellular anatomical entity; commissure; cell projection. The phenotypic classes of alleles include: neuroanatomy defective; oxidative stress response defective; viable; size defective; cell migration defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed at stages throughout embryogenesis.
Coordinates
chrX:14610435-14620888:-
Coord C1 exon
chrX:14620700-14620888
Coord A exon
chrX:14610661-14610810
Coord C2 exon
chrX:14610435-14610591
Length
150 bp
Sequences
Splice sites
3' ss Seq
ATTTCGCCCTTCATTTTCAGCCT
3' ss Score
8.75
5' ss Seq
TAAGTGAGT
5' ss Score
6.43
Exon sequences
Seq C1 exon
AGTGCAACTGCAACAAGCACGCCCGTCAGTGCCGCTTCAATATGGAAATCTTTCGGCTTTCGCAGGGCGTCTCCGGCGGAGTCTGCCAGAATTGTCGCCACTCGACTACGGGCCGGAATTGTCACCAGTGCAAGGAGGGATTCTATCGGGACGCCACCAAACCACTCACCCATCGCAAGGTTTGCAAAG
Seq A exon
CCTGCGATTGCCATCCGATCGGGTCATCTGGCAAGATCTGCAACAGCACCAGCGGTCAATGTCCCTGCAAGGACGGGGTCACCGGACTGACCTGCAACCGCTGCGCTCGAGGTTACCAGCAAAGTCGCTCCCACATCGCCCCCTGCATAA
Seq C2 exon
AACAACCACCGCGTATGATCAACATGCTGGACACCCAGAATACCGCTCCCGAGCCGGATGAGCCCGAATCCTCTCCAGGATCTGGTGGCGACCGCAACGGCGCCGCCGGAATGGCCGCCCAGTCTCAGTACTATCGCACCGAGGGCGGCAGGGGTAA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0015773-'1-3,'1-2,5-3
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact

Alternative protein isoforms (Ref)

No structure available
Features
Disorder rate (Iupred):
  C1=0.000 A=0.039 C2=0.878
Domain overlap (PFAM):

C1:
PF0005319=Laminin_EGF=WD(100=95.3)
A:
PF0005319=Laminin_EGF=WD(100=94.1)
C2:
NO


Main Inclusion Isoform:
FBpp0073759


Main Skipping Isoform:
NA


Other Inclusion Isoforms:
FBpp0300656, FBpp0311922


Other Skipping Isoforms:
NA
Associated events
Primers PCR
Suggestions for RT-PCR validation
F:
CCGTCAGTGCCGCTTCAATAT
R:
TGGAGAGGATTCGGGCTCATC
Band lengths:
244-394
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Neural diversity
  • Neurogenesis
  • Neuronal activity
  • Splicing factor regulation (brain)
  • Splicing factor regulation (SL2)