DmeEX6013554 @ dm6
Exon Skipping
Gene
FBgn0035050 | ST6Gal
Description
The gene Sialyltransferase is referred to in FlyBase by the symbol DmelSiaT (CG4871, FBgn0035050). It is a protein_coding_gene from Dmel. It has 3 annotated transcripts and 3 polypeptides (2 unique). Gene sequence location is 2R:24691422..24694386. Its molecular function is described by: sialyltransferase activity; beta-galactoside alpha-2,6-sialyltransferase activity. It is involved in the biological process described with: oligosaccharide metabolic process; neuromuscular junction development; protein glycosylation. 12 alleles are reported. The phenotypes of these alleles manifest in: embryonic/larval neuromuscular junction; wing; NMJ bouton. The phenotypic classes of alleles include: phenotype; fertile; paralytic; neurophysiology defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed during early larval stages, during late pupal stages.
Coordinates
chr2R:24691981-24693217:-
Coord C1 exon
chr2R:24692675-24693217
Coord A exon
chr2R:24692323-24692420
Coord C2 exon
chr2R:24691981-24692260
Length
98 bp
Sequences
Splice sites
3' ss Seq
ACGCTCCGCCCTGCTCCCAGACA
3' ss Score
7.39
5' ss Seq
CAAGTAGGC
5' ss Score
5.3
Exon sequences
Seq C1 exon
CGTGCGCTGGAACCCCAACGAGAAGTTCATCGTGGAGAGTCGCGAGAATCCGGCCATCAACTCGTCCAAGCTGGCTCCGCATCCCCGACTCAAGGTCTCGAAGAACACCAAGCTCACGCTGAGCCCCAAGCTGTATCTCTGCCACGACAAGCACTCGGAGCTGTGCCACAACAAGACCCAGCAGTTTCGCCAGCGCATTGTCCGGGCCTTCGAGAAGGCCATGGTGGAGTCGGTAAACGAGTCGCAGGCGAACCACTACAACGTGGACTACAAGCCCGTCTTTGGGGACAGCTTCGAGGAGCAGTACTACCCCTCGACCTGCCTGGTCATGGAGGCGGGCGTGCGGGTCCTGCGCCGCAAGGACGCGCCCTTCAACAAACTGCCCTTCGGCCGGCTCTTTCCGCGTCAAAAGCTCTTCCGCAACGTCAAAGACATCAAGACCTGCGCCATCGTCTCCAGTGCGGGATCCTTGGCTGGCTCCAAGTTAGGCCGCTTCATTG
Seq A exon
ACACGCACGACATTGTGATGAGATTCAATCATGCGCCCACGCAAGGACACGAGGTGGATGTTGGCAGCAAAACCACGATCCGTGTGGTGAATTCGCAA
Seq C2 exon
GTGGTCACCAAGCCGGAATTCGACTTCACCCGAGCCCCCATCTTCCGGAACGTCACGATCGCCGCCTGGGATCCGGGCAAGTACAATGGCACACTGGAGGATTGGCTCACTAGCGCTGACTACGACCTCTTTTCCAACTACGAGCTGTACAGGCGGCGCTATCCGAAGTCACGTGCCTTCCTCATCGATCCCCACTCCGTGTGGCGCCTGTGGCAGAGTCTGCAGATGTTTGCCGGAAACCGGCCGATAAGCAAGAATCCCCCCAGTTCCGGTTTTATAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0035050-'3-8,'3-7,4-8=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.171 A=0.030 C2=0.000
Domain overlap (PFAM):
C1:
PF0077713=Glyco_transf_29=PU(28.3=29.2)
A:
PF0077713=Glyco_transf_29=FE(11.9=100)
C2:
PF0077713=Glyco_transf_29=FE(34.6=100)
Main Inclusion Isoform:
FBpp0072346
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0072347
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
AAGCTCTTCCGCAACGTCAAA
R:
CATTGTACTTGCCCGGATCCC
Band lengths:
179-277
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)