GgaEX0028351 @ galGal4

Exon Skipping

Gene

ENSGALG00000007943 | lasp-2

Description

nebulette non-muscle isoform [Source:RefSeq peptide;Acc:NP_989819]

Coordinates

chr2:18207593-18225701:+

Coord C1 exon

chr2:18207593-18207703

Coord A exon

chr2:18210374-18210484

Coord C2 exon

chr2:18225530-18225701

Length

111 bp

Sequences

Splice sites

3' ss Seq

CATAAGGTTTTATCTTACAGAAA

3' ss Score

6.73

5' ss Seq

ACTGTAAGT

5' ss Score

8.59

Exon sequences

Seq C1 exon

AAAGAGTATAGAAAAGATTTGGAAGAATGGATGAAAGGAAAAGGAATGACAGTGTTTGAAGATACGCCAGATTTGATTAGAGTTAAAAATGCGGCTCAGATACTAAATGAG

Seq A exon

AAACAATACAAGAAAGACCTGGAAACTGAAATTAAAGGGAAGGGCATGCAAGTTGGTCCAGATACTCCTGAGATAAGACGAGCCAAGAAAGCTTCTGAAATTGCAAGCACT

Seq C2 exon

GTTAAATATCATGAAGATTTTGAGAAGACAAAGGGCAGGGGTTTCACGCCAGTGGTGGATGATCCTATAACAGAGCGGGTGCGGAAAAACACCCAAGTTGTAAGTGATGCAGCATATAAAGGGGTGCACCCACATATAGTTGAAATGGATAGAAGACCTGGAATAATTGTTG

VastDB Features

Vast-tools module Information

Secondary ID

ENSGALG00000007943_MULTIEX1-18/26=17-C2

Average complexity

C3

Mappability confidence:

89%=100=90%

Protein Impact

Alternative protein isoforms (No Ref)

No structure available

Features

Disorder rate (Iupred):

C1=0.000 A=0.730 C2=0.428

Domain overlap (PFAM):

C1:

PF0088013=Nebulin=PD(37.9=29.7),PF0088013=Nebulin=PU(55.2=43.2)

A:

PF0088013=Nebulin=PD(37.9=29.7),PF0088013=Nebulin=PU(55.2=43.2)

C2:

PF0088013=Nebulin=PD(37.9=19.0),PF0088013=Nebulin=WD(100=41.4)

Main Inclusion Isoform:

ENSGALP00000012888

Main Skipping Isoform:

ENSGALT00000012901fB1254

Other Inclusion Isoforms:

NA

Other Skipping Isoforms:

NA

Other assemblies

galGal3

Conservation

Human

(hg38)

chr10:20835513-20835623

ALTERNATIVE

HsaEX0042365

(NEBL)

Human

(hg19)

chr10:21124442-21124552

ALTERNATIVE

HsaEX0042365

(NEBL)

Mouse

(mm10)

chr2:17397057-17397167

ALTERNATIVE

MmuEX0031353

(Nebl)

Mouse

(mm9)

chr2:17318684-17318794

ALTERNATIVE

MmuEX0031353

(Nebl)

Rat

(rn6)

chr17:84186037-84186147

ALTERNATIVE

RnoEX0059036

(Nebl)

Cow

(bosTau6)

chr13:22441343-22441453

ALTERNATIVE

BtaEX0024198

(NEBL)

Zebrafish

(danRer10)

chr9:23013261-23013371

ALTERNATIVE

DreEX0050011

(neb)

Fruitfly

(dm6)

No conservation detected

Primers PCR

Suggestions for RT-PCR validation

F:

TTGGAAGAATGGATGAAAGGAAAAGG

R:

TCCATTTCAACTATATGTGGGTGCA

Band lengths:

242-353

Functional annotations

(Submit an annotation)

There are 0 annotated functions for this event

GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]

Special

GgaEX0028351 @ galGal4

GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]