GgaEX0028353 @ galGal4

Exon Skipping

Gene

ENSGALG00000007943 | lasp-2

Description

nebulette non-muscle isoform [Source:RefSeq peptide;Acc:NP_989819]

Coordinates

chr2:18211125-18225701:+

Coord C1 exon

chr2:18211125-18211235

Coord A exon

chr2:18211563-18211673

Coord C2 exon

chr2:18225530-18225701

Length

111 bp

Sequences

Splice sites

3' ss Seq

TAATTTATTTTATTTTGCAGAAA

3' ss Score

9.37

5' ss Seq

CAGGTAGGC

5' ss Score

9.88

Exon sequences

Seq C1 exon

AAAGAGTATAAGAAAGACTTGGAGAATGAAATTAAAGGAAAGGGAATGGAAGTGGGCATGGATACCCCTGATATACAACGAGCAAAGAAAGCTTCTGAAATTGTAAGCCAG

Seq A exon

AAAGAATATAGAAAAGATCTGGAGACTGAAATTATAGGAAAAGGGATGCAAGTTGGTCCATTTACACCTGAAATACAACGTGTCAAAAGAGCTTCAGAAATAGCAAGCCAG

Seq C2 exon

GTTAAATATCATGAAGATTTTGAGAAGACAAAGGGCAGGGGTTTCACGCCAGTGGTGGATGATCCTATAACAGAGCGGGTGCGGAAAAACACCCAAGTTGTAAGTGATGCAGCATATAAAGGGGTGCACCCACATATAGTTGAAATGGATAGAAGACCTGGAATAATTGTTG

VastDB Features

Vast-tools module Information

Secondary ID

ENSGALG00000007943_MULTIEX1-20/26=19-C2

Average complexity

C3

Mappability confidence:

86%=100=75%

Protein Impact

Alternative protein isoforms (No Ref)

No structure available

Features

Disorder rate (Iupred):

C1=0.811 A=0.486 C2=0.428

Domain overlap (PFAM):

C1:

PF0088013=Nebulin=PD(37.9=29.7),PF0088013=Nebulin=PU(57.1=43.2)

A:

PF0088013=Nebulin=PD(35.7=27.0),PF0088013=Nebulin=PU(57.1=43.2)

C2:

PF0088013=Nebulin=PD(37.9=19.0),PF0088013=Nebulin=WD(100=41.4)

Main Inclusion Isoform:

ENSGALP00000012888

Main Skipping Isoform:

ENSGALT00000012901fB1254

Other Inclusion Isoforms:

NA

Other Skipping Isoforms:

NA

Other assemblies

galGal3

Conservation

Human

(hg38)

chr10:20831533-20831579

ALTERNATIVE

HsaEX0042366

(NEBL)

Human

(hg19)

chr10:21120402-21120512

ALTERNATIVE

HsaEX0042366

(NEBL)

Mouse

(mm10)

chr2:17393166-17393276

ALTERNATIVE

MmuEX0031354

(Nebl)

Mouse

(mm9)

chr2:17314793-17314903

ALTERNATIVE

MmuEX0031354

(Nebl)

Rat

(rn6)

chr17:84182249-84182359

ALTERNATIVE

RnoEX0059037

(Nebl)

Cow

(bosTau6)

chr13:22433394-22433504

ALTERNATIVE

BtaEX0024199

(NEBL)

Zebrafish

(danRer10)

chr9:23011116-23011229

HIGH PSI

DreEX0050012

(neb)

Fruitfly

(dm6)

No conservation detected

Primers PCR

Suggestions for RT-PCR validation

F:

GGAAAGGGAATGGAAGTGGGC

R:

ACAATTATTCCAGGTCTTCTATCCA

Band lengths:

245-356

Functional annotations

(Submit an annotation)

There are 0 annotated functions for this event

GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]

Special

GgaEX0028353 @ galGal4

GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]